Detailed information of TA system TA085932

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 41590..42215
• Replicon: plasmid pEh-Pc1
• Accession: NZ_CP023707
• Organism: Edwardsiella tarda strain KC-Pc-HB1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CPU03_RS17080
• Protein ID: WP_097364766.1
• Coordinates: 41817..42215 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CPU03_RS17075
• Protein ID: WP_068872245.1
• Coordinates: 41590..41817 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPU03_RS17075	41590..41817	+	228	WP_068872245.1	antitoxin	Antitoxin
CPU03_RS17080	41817..42215	+	399	WP_097364766.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CPU03_RS17085	42293..44497	-	2205	WP_097364767.1	type IV conjugative transfer system coupling protein TraD	-
CPU03_RS17090	44584..45081	-	498	WP_097364768.1	conjugal transfer protein TraS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	faeJ / faeI / faeH / faeG / faeG / faeF / faeE / faeD / faeC	1..81404	81404

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-32)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14882.24 Da
• Isoelectric Point: 8.5070

>T85932 WP_097364766.1 NZ_CP023707:41817-42215 [Edwardsiella tarda]
MLKYMLDTNICIFTLKNRPAQVREKFNLHHNQMCISAITLMELIYGAEKSQAPERNLAVIEGFIARLTVLNYDAPAAAHS
GQLRAEQARLGQPIGPYDQMIAGHARSQGLILVTNNTREFMRVSGLRVEDWV

Nucleotide

• Length: 399 bp

>T85932 NZ_CP023707:41817-42215 [Edwardsiella tarda]
ATGCTGAAATACATGCTGGATACCAACATTTGCATCTTCACACTGAAAAACAGACCCGCCCAGGTTCGAGAGAAATTTAA
CCTGCACCATAATCAGATGTGTATCAGCGCGATTACCCTGATGGAGCTGATCTATGGTGCGGAGAAAAGCCAAGCCCCTG
AGCGTAATCTGGCGGTTATTGAAGGATTTATTGCCCGCTTGACTGTATTGAACTACGACGCCCCGGCAGCCGCTCACTCC
GGCCAGCTGCGCGCAGAGCAAGCCAGGCTGGGTCAACCGATTGGCCCTTATGATCAGATGATCGCAGGCCACGCTCGTAG
CCAGGGATTGATCCTCGTCACCAATAATACCCGCGAGTTTATGCGTGTCTCCGGGCTGAGAGTTGAAGATTGGGTGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8307.28 Da
• Isoelectric Point: 4.5203

>AT85932 WP_068872245.1 NZ_CP023707:41590-41817 [Edwardsiella tarda]
METTVFLSNRSQAVRLPKAVALPGDVKRVDVVAVGRTRIIAPAGESWDNWFEGEGVSADFMASREQPQGQEREDF

Nucleotide

• Length: 228 bp

>AT85932 NZ_CP023707:41590-41817 [Edwardsiella tarda]
ATGGAAACAACCGTTTTTCTCAGCAACCGCAGCCAGGCTGTCAGATTGCCAAAGGCCGTTGCATTGCCCGGTGATGTCAA
ACGGGTCGATGTGGTTGCCGTTGGACGAACCCGCATCATTGCGCCCGCAGGCGAATCCTGGGATAACTGGTTTGAGGGTG
AAGGCGTCAGTGCGGATTTTATGGCTAGCAGAGAGCAGCCGCAGGGACAGGAGCGTGAGGATTTCTGA