Detailed information of TA system TA085919

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 666887..667479
• Replicon: chromosome
• Accession: NZ_CP023706
• Organism: Edwardsiella tarda strain KC-Pc-HB1

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D4F316
• Locus tag: CPU03_RS03265
• Protein ID: WP_005283517.1
• Coordinates: 667276..667479 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: D4F317
• Locus tag: CPU03_RS03260
• Protein ID: WP_005283520.1
• Coordinates: 666887..667255 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPU03_RS03250	662129..663313	+	1185	WP_005296656.1	efflux RND transporter periplasmic adaptor subunit	-
CPU03_RS03255	663358..666513	+	3156	WP_005283526.1	multidrug efflux RND transporter permease subunit	-
CPU03_RS03260	666887..667255	+	369	WP_005283520.1	Hha toxicity modulator TomB	Antitoxin
CPU03_RS03265	667276..667479	+	204	WP_005283517.1	hemolysin expression modulator Hha	Toxin
CPU03_RS03280	668547..668864	+	318	WP_035600211.1	MGMT family protein	-
CPU03_RS03285	668945..669811	+	867	WP_068869964.1	acyl-CoA thioesterase II	-
CPU03_RS03290	669836..671113	-	1278	WP_035594812.1	ammonium transporter AmtB	-
CPU03_RS03295	671140..671478	-	339	WP_035594810.1	P-II family nitrogen regulator	-
CPU03_RS03300	671758..672219	-	462	WP_005296844.1	Lrp/AsnC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-115)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8088.36 Da
• Isoelectric Point: 5.8288

>T85919 WP_005283517.1 NZ_CP023706:667276-667479 [Edwardsiella tarda]
MTKIDYLMKLRKCTSLETLERVIEKNKYELSNDELEVFYSAADHRLAELTMNKLYDKIPAEVWQYVR

Nucleotide

• Length: 204 bp

>T85919 NZ_CP023706:667276-667479 [Edwardsiella tarda]
ATGACCAAAATAGACTATCTGATGAAGTTGCGTAAGTGCACGTCGCTGGAGACGCTAGAACGCGTGATCGAAAAGAATAA
ATATGAGCTTTCCAATGATGAGCTGGAAGTTTTTTATTCCGCAGCCGATCATCGCTTAGCCGAATTAACTATGAATAAGC
TATACGATAAAATTCCAGCCGAAGTCTGGCAGTACGTACGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14260.05 Da
• Isoelectric Point: 6.0582

>AT85919 WP_005283520.1 NZ_CP023706:666887-667255 [Edwardsiella tarda]
MDEYTSYRHDIAELKYLCDSLYRQGMDVLEESHHGWVSDPTAMVNLQLNELIEHIANVALSFKIKYPQHSSLCEIVDDYL
DETYALFSAYSVSEQALRHWLRSKRRVAYSLAHEKRNAALHV

Nucleotide

• Length: 369 bp

>AT85919 NZ_CP023706:666887-667255 [Edwardsiella tarda]
ATGGATGAGTACACATCTTATCGACATGATATCGCAGAGTTAAAATATCTGTGTGATTCCCTGTATCGTCAGGGAATGGA
TGTCCTGGAAGAGAGTCATCATGGCTGGGTCAGCGATCCGACGGCAATGGTTAATCTTCAGCTTAATGAGCTGATTGAGC
ATATTGCCAACGTGGCCTTGTCATTTAAGATCAAATATCCACAGCATTCGTCTCTTTGCGAAATAGTGGATGATTATCTG
GATGAAACCTATGCCTTGTTCAGCGCCTATAGCGTGAGTGAACAGGCACTCAGGCACTGGTTACGAAGCAAGCGCAGAGT
TGCGTACTCGCTCGCACATGAGAAACGTAACGCCGCCTTGCATGTTTAA

Structures

Toxin

• AlphaFold DB: D4F316

Antitoxin

• AlphaFold DB: D4F317