Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 2129235..2130745 Replicon chromosome
Accession NZ_CP023705
Organism Sphingomonas melonis strain ZJ26

Toxin (Protein)


Gene name HipA1 Uniprot ID A0A175Y3U4
Locus tag CP552_RS09920 Protein ID WP_017980415.1
Coordinates 2129235..2130500 (-) Length 422 a.a.

Antitoxin (Protein)


Gene name HipB1 Uniprot ID A0A175Y469
Locus tag CP552_RS09925 Protein ID WP_017980416.1
Coordinates 2130497..2130745 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP552_RS09900 2124818..2125717 + 900 WP_017980411.1 HNH endonuclease -
CP552_RS09905 2126063..2126513 + 451 Protein_1958 glutathione S-transferase C-terminal domain-containing protein -
CP552_RS09910 2126519..2127403 - 885 WP_017980413.1 LysR family transcriptional regulator -
CP552_RS09915 2127503..2129017 + 1515 WP_172436203.1 MFS transporter -
CP552_RS09920 2129235..2130500 - 1266 WP_017980415.1 type II toxin-antitoxin system HipA family toxin Toxin
CP552_RS09925 2130497..2130745 - 249 WP_017980416.1 helix-turn-helix transcriptional regulator Antitoxin
CP552_RS09930 2131191..2132462 + 1272 WP_017980418.1 dicarboxylate/amino acid:cation symporter -
CP552_RS09935 2132644..2134425 - 1782 WP_017980419.1 hypothetical protein -
CP552_RS09940 2134485..2134916 + 432 WP_017980420.1 GtrA family protein -
CP552_RS09945 2134920..2135648 + 729 WP_017980421.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-75)


Sequences

Toxin        


Download         Length: 422 a.a.        Molecular weight: 46236.14 Da        Isoelectric Point: 6.4978

>T85918 WP_017980415.1 NZ_CP023705:c2130500-2129235 [Sphingomonas melonis]
MILPVYFEEHLVGEVHEVVDGPRFVYAASWTEDRQAFPISITMPLGEREVEPARFLTWASNLLPEAEQLVVIARQLGVAQ
TDILALLEAIGRDTAGALSFGAPGLTRTERWQRIETEDDLERIIEELPRKPFLAGDDGVSMSLAGVQTKLAVACDADGRI
CIPLDGSPSTHILKPDSERLHGSVQNEAWCLTLARLCGLTVPPVTTGVAGGRRYFLIPRYDRVPDGAQWRRLHQEDFCQA
LGKPPFAKYERNRTGVRGPTVADMMNLVRRQALGPDALRLFDAIVFNVLACNTDAHAKNYSILIPAGSRVRTAPLYDVMC
AEVFDGVTHNLAQTVADKNRGNYLKRRHWLRLAAACGLGAAPTIRRVRALATRVLANVDRARMDVEAMPAGGDATLPMIV
DAVSERCRAILAGLDDMEGVK

Download         Length: 1266 bp

>T85918 NZ_CP023705:c2130500-2129235 [Sphingomonas melonis]
ATGATCCTCCCGGTCTATTTTGAGGAGCATCTGGTCGGCGAAGTCCATGAGGTCGTTGACGGACCGCGGTTCGTGTACGC
AGCATCGTGGACGGAGGACCGACAGGCCTTCCCCATCTCGATCACCATGCCGCTGGGCGAGCGCGAGGTGGAGCCTGCTC
GGTTCCTGACCTGGGCCAGCAACCTGCTTCCCGAGGCGGAGCAGCTCGTGGTCATCGCCCGTCAACTCGGTGTCGCGCAG
ACAGACATCCTCGCCCTGCTGGAGGCGATCGGCCGCGACACCGCCGGCGCGCTTTCCTTTGGTGCGCCGGGGCTGACCCG
CACAGAGCGCTGGCAGCGGATCGAGACAGAGGACGATCTCGAACGCATCATCGAGGAGCTTCCCCGCAAGCCGTTCCTCG
CGGGCGATGACGGCGTGTCGATGTCGCTGGCGGGCGTCCAGACCAAGCTGGCGGTGGCCTGCGACGCCGACGGCCGGATC
TGCATTCCGCTCGACGGGTCGCCCTCGACCCATATCCTCAAGCCCGACAGCGAACGCCTCCACGGCAGCGTCCAGAACGA
GGCTTGGTGCCTGACGCTGGCTAGGCTCTGCGGATTGACGGTGCCGCCGGTTACGACCGGAGTGGCGGGCGGAAGGCGCT
ACTTCCTGATCCCGCGCTACGACCGCGTGCCAGACGGAGCGCAATGGCGTCGGCTGCATCAGGAGGACTTTTGCCAAGCG
CTGGGTAAGCCACCGTTCGCCAAGTACGAGCGGAACAGAACGGGCGTCCGTGGCCCGACCGTCGCCGACATGATGAACCT
CGTCCGGCGCCAGGCGCTCGGTCCTGATGCACTGCGGCTGTTCGACGCCATCGTCTTCAACGTACTGGCGTGCAACACCG
ATGCCCATGCCAAGAACTACTCGATCCTGATCCCCGCAGGATCGCGGGTGCGCACCGCTCCCCTATACGACGTGATGTGC
GCGGAGGTGTTCGATGGCGTGACCCACAATCTCGCGCAGACGGTCGCCGACAAGAACCGCGGCAACTATCTGAAGCGAAG
GCACTGGCTGCGCCTCGCCGCCGCCTGCGGACTCGGCGCTGCACCGACCATCCGCCGCGTCCGCGCGCTGGCGACCCGGG
TGCTCGCCAATGTCGATCGGGCGCGGATGGATGTCGAAGCCATGCCCGCAGGCGGTGACGCCACCCTGCCAATGATCGTC
GACGCCGTGTCGGAGCGGTGCCGCGCTATCCTCGCTGGTCTCGATGACATGGAAGGCGTGAAGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8897.16 Da        Isoelectric Point: 5.7710

>AT85918 WP_017980416.1 NZ_CP023705:c2130745-2130497 [Sphingomonas melonis]
MDQADTENDAAWFSRRFGALVRSRRQQMGLSLEDLATVAGVGIRFIHELEKGKPTCQIGRALVVAGLVGLDPVTLLEQQS
AS

Download         Length: 249 bp

>AT85918 NZ_CP023705:c2130745-2130497 [Sphingomonas melonis]
ATGGATCAGGCTGACACGGAAAACGATGCCGCATGGTTTTCGCGCCGCTTCGGTGCCCTCGTCCGCTCGCGCCGACAGCA
GATGGGGCTAAGTCTCGAGGATCTGGCGACCGTCGCGGGCGTGGGTATCCGGTTCATCCACGAATTGGAGAAGGGCAAGC
CGACCTGCCAGATCGGCCGCGCCCTCGTCGTCGCCGGGCTGGTCGGGCTGGACCCGGTCACCCTGCTTGAACAGCAAAGC
GCCTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A175Y3U4

Antitoxin

Source ID Structure
AlphaFold DB A0A175Y469

References