Detailed information of TA system TA085917

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/PRK09907-MazE
• Location: 2063198..2063808
• Replicon: chromosome
• Accession: NZ_CP023704
• Organism: Caldibacillus thermoamylovorans strain SSBM

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CQJ30_RS10075
• Protein ID: WP_108899113.1
• Coordinates: 2063198..2063533 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A2U8EC75
• Locus tag: CQJ30_RS10080
• Protein ID: WP_034770392.1
• Coordinates: 2063533..2063808 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQJ30_RS10050	2058950..2059621	-	672	WP_108898086.1	ABC transporter permease	-
CQJ30_RS10055	2059621..2060544	-	924	WP_192878682.1	osmoprotectant ABC transporter substrate-binding protein	-
CQJ30_RS10060	2060561..2061211	-	651	WP_095142396.1	ABC transporter permease	-
CQJ30_RS10065	2061228..2062370	-	1143	WP_108898087.1	betaine/proline/choline family ABC transporter ATP-binding protein	-
CQJ30_RS10075	2063198..2063533	-	336	WP_108899113.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CQJ30_RS10080	2063533..2063808	-	276	WP_034770392.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CQJ30_RS10085	2063925..2064416	-	492	WP_108898088.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
CQJ30_RS10090	2064649..2065536	-	888	WP_108898089.1	fructose bisphosphate aldolase	-
CQJ30_RS10095	2065905..2066840	-	936	WP_108898090.1	EamA family transporter RarD	-
CQJ30_RS10100	2067205..2067744	+	540	WP_041847840.1	HD domain-containing protein	-
CQJ30_RS10105	2068058..2068237	-	180	WP_034770399.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-100)

Antitoxin

• SpoVT-AbrB domain (18-62)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12471.30 Da
• Isoelectric Point: 6.4491

>T85917 WP_108899113.1 NZ_CP023704:c2063533-2063198 [Caldibacillus thermoamylovorans]
MNQTPDRGDIVYLNFSPQSGPEQAGHRPAIVLSPKLFNSGTFAVVCPITTKEKGYPFEVKIPDKLNVQGVILTDQLRSVD
WRSRNLKIVDHVPDEVVQNCLDLVSTFLPFY

Nucleotide

• Length: 336 bp

>T85917 NZ_CP023704:c2063533-2063198 [Caldibacillus thermoamylovorans]
ATGAATCAAACACCAGACAGAGGCGATATAGTTTATCTTAATTTCAGTCCTCAATCCGGTCCTGAACAGGCAGGACATAG
ACCGGCTATTGTTTTATCACCCAAATTATTTAACAGTGGAACTTTTGCAGTTGTATGTCCAATCACGACTAAAGAAAAAG
GCTATCCTTTCGAAGTTAAAATACCAGATAAATTGAATGTTCAAGGGGTAATTTTAACCGATCAATTAAGGAGTGTTGAT
TGGCGTTCAAGAAATTTAAAGATTGTGGACCATGTTCCAGATGAAGTTGTTCAGAATTGCCTAGATTTAGTATCCACATT
TTTACCTTTTTATTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10363.08 Da
• Isoelectric Point: 9.6879

>AT85917 WP_034770392.1 NZ_CP023704:c2063808-2063533 [Caldibacillus thermoamylovorans]
IKMNKENKGEIKMTTKVQRWGNSLAVRLPKVILDDLNIKEGSDISISVENNAIKIIPIKKKPTLEELMSKITPENKHDEV
NWGKPEGAEIW

Nucleotide

• Length: 276 bp

>AT85917 NZ_CP023704:c2063808-2063533 [Caldibacillus thermoamylovorans]
ATTAAAATGAATAAAGAAAACAAAGGGGAAATCAAGATGACCACAAAAGTGCAAAGATGGGGAAATAGTCTTGCTGTTAG
ACTTCCGAAAGTTATATTAGACGACTTAAATATTAAAGAAGGTTCAGACATTAGTATTTCTGTAGAAAATAATGCAATCA
AAATTATTCCGATAAAGAAAAAACCAACTCTTGAAGAATTAATGTCAAAAATTACTCCTGAAAATAAACATGATGAAGTG
AATTGGGGCAAACCAGAAGGTGCTGAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2U8EC75