Detailed information of TA system TA085893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 69158..69717
• Replicon: plasmid pO177A2
• Accession: NZ_CP023674
• Organism: Escherichia coli strain SMN013SH2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: CP957_RS28385
• Protein ID: WP_000421257.1
• Coordinates: 69442..69717 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CP957_RS28380
• Protein ID: WP_062881718.1
• Coordinates: 69158..69442 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP957_RS28330	64337..64897	+	561	WP_101974184.1	fertility inhibition protein FinO	-
CP957_RS28335	65033..65245	+	213	WP_001299730.1	ANR family transcriptional regulator	-
CP957_RS29555	65491..65565	+	75	Protein_65	endonuclease	-
-	65821..65882	-	62	NuclAT_0	-	-
-	65821..65882	-	62	NuclAT_0	-	-
-	65821..65882	-	62	NuclAT_0	-	-
-	65821..65882	-	62	NuclAT_0	-	-
CP957_RS28345	65869..66075	+	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	-
CP957_RS28350	66359..66616	+	258	WP_000083845.1	replication regulatory protein RepA	-
CP957_RS28360	66848..66922	+	75	WP_001442103.1	RepA leader peptide Tap	-
CP957_RS28365	66915..67397	+	483	WP_001523473.1	hypothetical protein	-
CP957_RS28370	67390..68247	+	858	WP_096851680.1	incFII family plasmid replication initiator RepA	-
CP957_RS28380	69158..69442	+	285	WP_062881718.1	ribbon-helix-helix domain-containing protein	Antitoxin
CP957_RS28385	69442..69717	+	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	espP / hlyD / hlyB / hlyA / hlyC	1..87524	87524

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T85893 WP_000421257.1 NZ_CP023674:69442-69717 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T85893 NZ_CP023674:69442-69717 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6471

>AT85893 WP_062881718.1 NZ_CP023674:69158-69442 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLDALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT85893 NZ_CP023674:69158-69442 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGATGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAAGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: S1PMX5