Detailed information of TA system TA085884

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3915003..3915804
• Replicon: chromosome
• Accession: NZ_CP023673
• Organism: Escherichia coli strain SMN013SH2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: CP957_RS21490
• Protein ID: WP_001094440.1
• Coordinates: 3915003..3915380 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CP957_RS21495
• Protein ID: WP_024166067.1
• Coordinates: 3915427..3915804 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP957_RS21460	3910533..3911852	+	1320	WP_000144685.1	site-specific integrase	-
CP957_RS21465	3911945..3912793	-	849	WP_001280444.1	DUF4942 domain-containing protein	-
CP957_RS21475	3913076..3914095	-	1020	WP_000875212.1	IS110 family transposase	-
CP957_RS21480	3914309..3914506	-	198	WP_000839232.1	DUF957 domain-containing protein	-
CP957_RS21485	3914518..3915006	-	489	WP_000761703.1	hypothetical protein	-
CP957_RS21490	3915003..3915380	-	378	WP_001094440.1	TA system toxin CbtA family protein	Toxin
CP957_RS21495	3915427..3915804	-	378	WP_024166067.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CP957_RS21500	3915879..3916100	-	222	WP_000692350.1	DUF987 domain-containing protein	-
CP957_RS21505	3916187..3916663	-	477	WP_001367144.1	RadC family protein	-
CP957_RS21510	3916679..3917158	-	480	WP_001449312.1	antirestriction protein	-
CP957_RS21515	3917252..3917497	-	246	WP_000680583.1	hypothetical protein	-
CP957_RS21520	3917497..3918315	-	819	WP_001234621.1	DUF945 domain-containing protein	-
CP957_RS21530	3918536..3918946	-	411	WP_000846706.1	hypothetical protein	-
CP957_RS21535	3918962..3919645	-	684	WP_000775504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	sul2 / aph(6)-Id / aph(3'')-Ib / tet(B)	fdeC / yagZ/ecpA / yagY/ecpB / yagX/ecpC	3890962..3961251	70289
	-	flank	IS/Tn	-	-	3913076..3914095	1019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13961.87 Da
• Isoelectric Point: 7.3225

>T85884 WP_001094440.1 NZ_CP023673:c3915380-3915003 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRSHYRTVNDITLGKRTEAKR

Nucleotide

• Length: 378 bp

>T85884 NZ_CP023673:c3915380-3915003 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGTCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CCACTACAGAACGGTAAATGACATTACCCTGGGTAAACGCACGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13676.53 Da
• Isoelectric Point: 5.9505

>AT85884 WP_024166067.1 NZ_CP023673:c3915804-3915427 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Nucleotide

• Length: 378 bp

>AT85884 NZ_CP023673:c3915804-3915427 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCCGACTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCGGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA