Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2445359..2445997 Replicon chromosome
Accession NZ_CP023673
Organism Escherichia coli strain SMN013SH2

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag CP957_RS13460 Protein ID WP_032195248.1
Coordinates 2445359..2445535 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag CP957_RS13465 Protein ID WP_077879189.1
Coordinates 2445581..2445997 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP957_RS13440 2440978..2442192 - 1215 WP_071796541.1 BenE family transporter YdcO -
CP957_RS13445 2442245..2442781 + 537 WP_062881684.1 DNA-binding transcriptional regulator SutR -
CP957_RS13450 2442854..2444815 + 1962 WP_024177737.1 23S rRNA 5-hydroxycytidine C2501 synthase -
CP957_RS13455 2444907..2445137 - 231 WP_000494244.1 YncJ family protein -
CP957_RS13460 2445359..2445535 + 177 WP_032195248.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
CP957_RS13465 2445581..2445997 + 417 WP_077879189.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
CP957_RS13470 2446076..2447482 + 1407 WP_000760615.1 PLP-dependent aminotransferase family protein -
CP957_RS13475 2447727..2448872 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
CP957_RS13480 2448924..2449901 + 978 WP_095585825.1 ABC transporter ATP-binding protein -
CP957_RS13485 2449902..2450843 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleF / espM1 2397210..2449901 52691


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6709.81 Da        Isoelectric Point: 11.2298

>T85878 WP_032195248.1 NZ_CP023673:2445359-2445535 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRPPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T85878 NZ_CP023673:2445359-2445535 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCCCCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15232.60 Da        Isoelectric Point: 4.7386

>AT85878 WP_077879189.1 NZ_CP023673:2445581-2445997 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPVSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT85878 NZ_CP023673:2445581-2445997 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTGTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References