Detailed information of TA system TA085869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1287677..1288302
• Replicon: chromosome
• Accession: NZ_CP023673
• Organism: Escherichia coli strain SMN013SH2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CP957_RS06815
• Protein ID: WP_000911330.1
• Coordinates: 1287904..1288302 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: CP957_RS06810
• Protein ID: WP_000450524.1
• Coordinates: 1287677..1287904 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP957_RS06785	1283479..1283949	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
CP957_RS06790	1283949..1284521	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
CP957_RS06795	1284667..1285545	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CP957_RS06800	1285562..1286596	+	1035	WP_062882059.1	outer membrane protein assembly factor BamC	-
CP957_RS06805	1286809..1287522	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CP957_RS06810	1287677..1287904	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CP957_RS06815	1287904..1288302	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CP957_RS06820	1288449..1289312	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
CP957_RS06825	1289327..1291342	+	2016	WP_062882060.1	tRNA cytosine(34) acetyltransferase TmcA	-
CP957_RS06830	1291416..1292018	+	603	Protein_1240	esterase	-
CP957_RS06835	1292128..1292328	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T85869 WP_000911330.1 NZ_CP023673:1287904-1288302 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T85869 NZ_CP023673:1287904-1288302 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT85869 WP_000450524.1 NZ_CP023673:1287677-1287904 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT85869 NZ_CP023673:1287677-1287904 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33