Detailed information of TA system TA085863

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 292951..293582
• Replicon: chromosome
• Accession: NZ_CP023673
• Organism: Escherichia coli strain SMN013SH2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CP957_RS01485
• Protein ID: WP_032203651.1
• Coordinates: 292951..293226 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: CP957_RS01490
• Protein ID: WP_000593555.1
• Coordinates: 293223..293582 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP957_RS01470	287955..289022	+	1068	WP_000361467.1	HlyD family secretion protein	-
CP957_RS01475	289019..291754	+	2736	WP_000149164.1	ribosome-associated ATPase/putative transporter RbbA	-
CP957_RS01480	291754..292878	+	1125	WP_001216257.1	ABC transporter permease	-
CP957_RS01485	292951..293226	+	276	WP_032203651.1	type II toxin-antitoxin system HicA family toxin	Toxin
CP957_RS01490	293223..293582	+	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CP957_RS01495	293702..294103	-	402	WP_062882695.1	nickel-responsive transcriptional regulator NikR	-
CP957_RS01500	294109..294915	-	807	WP_062882694.1	nickel import ATP-binding protein NikE	-
CP957_RS01505	294912..295676	-	765	WP_062882693.1	nickel import ATP-binding protein NikD	-
CP957_RS01510	295676..296509	-	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
CP957_RS01515	296506..297450	-	945	WP_000947068.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	285193..294103	8910

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10213.97 Da
• Isoelectric Point: 10.7076

>T85863 WP_032203651.1 NZ_CP023673:292951-293226 [Escherichia coli]
MRTKLQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T85863 NZ_CP023673:292951-293226 [Escherichia coli]
ATGAGAACCAAGTTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT85863 WP_000593555.1 NZ_CP023673:293223-293582 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT85863 NZ_CP023673:293223-293582 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LKZ6