Detailed information of TA system TA085862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 80676..81312
• Replicon: plasmid pLM6
• Accession: NZ_CP023670
• Organism: Methylomonas koyamae strain LM6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MKLM6_RS22345
• Protein ID: WP_096876862.1
• Coordinates: 80896..81312 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MKLM6_RS22340
• Protein ID: WP_096876861.1
• Coordinates: 80676..80909 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKLM6_RS22330	77441..80053	+	2613	WP_096876930.1	AAA family ATPase	-
MKLM6_RS22335	80279..80427	-	149	Protein_74	cold shock domain-containing protein	-
MKLM6_RS22340	80676..80909	+	234	WP_096876861.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MKLM6_RS22345	80896..81312	+	417	WP_096876862.1	PIN domain-containing protein	Toxin
MKLM6_RS22350	81508..82545	-	1038	WP_157770374.1	hypothetical protein	-
MKLM6_RS22355	82517..83929	-	1413	WP_096876864.1	MBOAT family protein	-
MKLM6_RS22360	83946..84308	-	363	WP_096876865.1	hypothetical protein	-
MKLM6_RS22365	84305..84796	-	492	WP_096876866.1	thermonuclease family protein	-
MKLM6_RS22370	84811..85443	-	633	WP_096876867.1	DUF4400 domain-containing protein	-
MKLM6_RS22375	85446..85844	-	399	WP_157770375.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..186658	186658

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-116)

Antitoxin

• SpoVT-AbrB domain (5-39)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15098.32 Da
• Isoelectric Point: 4.3875

>T85862 WP_096876862.1 NZ_CP023670:80896-81312 [Methylomonas koyamae]
MKTAKPFLDTNVLLYLLSEDNQKADRAEEIIAFGGVINVQVLNEFASVASRKLKMTYVEIRETLSVIRSICETQALSVSI
HEQGLAIAEQFGFSLYDSMIVSAALDAGCSILYSEDMQHGQAIGSLVINNPFLPSLPN

Nucleotide

• Length: 417 bp

>T85862 NZ_CP023670:80896-81312 [Methylomonas koyamae]
ATGAAAACCGCTAAGCCGTTTCTCGATACCAACGTCTTGCTGTATTTACTGTCTGAGGACAATCAAAAAGCAGACCGCGC
CGAAGAGATTATCGCCTTCGGCGGAGTAATCAATGTGCAGGTGTTGAATGAATTTGCGTCGGTAGCGTCGCGCAAGCTGA
AGATGACGTATGTCGAAATCCGCGAAACCTTGTCGGTTATCCGAAGTATCTGTGAAACTCAAGCTTTGAGCGTCAGCATC
CACGAACAAGGTTTGGCGATTGCCGAACAATTTGGTTTTTCGTTGTATGACAGCATGATTGTCAGCGCGGCGCTGGACGC
CGGGTGCTCAATATTGTATTCGGAAGACATGCAACATGGTCAAGCTATTGGCTCGTTAGTCATTAACAATCCATTTTTAC
CTTCACTTCCTAACTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8770.07 Da
• Isoelectric Point: 6.4683

>AT85862 WP_096876861.1 NZ_CP023670:80676-80909 [Methylomonas koyamae]
MQVAKWGNSLAIRLPATVVEALELKEGDDIEVHIVGERVFELDKKPSAQTLLAKLHQFRGRLPADFKFDRLEANENR

Nucleotide

• Length: 234 bp

>AT85862 NZ_CP023670:80676-80909 [Methylomonas koyamae]
ATGCAAGTCGCAAAATGGGGTAATAGTTTAGCTATTCGTTTGCCCGCCACGGTGGTAGAAGCGTTAGAGTTAAAGGAAGG
CGATGACATTGAAGTACATATCGTCGGCGAACGGGTATTTGAACTAGATAAAAAACCTTCGGCACAGACGTTGTTAGCCA
AATTGCATCAATTTCGTGGGCGCCTACCCGCTGATTTTAAGTTTGACCGCCTGGAAGCGAATGAAAACCGCTAA