Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 18930..19516 Replicon plasmid pLM6
Accession NZ_CP023670
Organism Methylomonas koyamae strain LM6

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag MKLM6_RS22095 Protein ID WP_096876826.1
Coordinates 18930..19268 (-) Length 113 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag MKLM6_RS22100 Protein ID WP_096876827.1
Coordinates 19268..19516 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKLM6_RS23280 14712..14921 + 210 WP_157770362.1 hypothetical protein -
MKLM6_RS22065 14892..15182 - 291 WP_157770363.1 hypothetical protein -
MKLM6_RS22070 15293..15529 + 237 WP_054758403.1 hypothetical protein -
MKLM6_RS23285 15544..15774 - 231 WP_054758400.1 hypothetical protein -
MKLM6_RS22075 16084..17082 - 999 WP_096876823.1 YqaJ viral recombinase family protein -
MKLM6_RS22080 17079..17426 - 348 WP_082889552.1 hypothetical protein -
MKLM6_RS22085 17517..18482 - 966 WP_096876824.1 phage recombination protein Bet -
MKLM6_RS22095 18930..19268 - 339 WP_096876826.1 endoribonuclease MazF Toxin
MKLM6_RS22100 19268..19516 - 249 WP_096876827.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
MKLM6_RS22105 19855..20760 + 906 WP_096876828.1 DNA-binding protein -
MKLM6_RS22110 21103..21999 - 897 WP_096876928.1 poly(3-hydroxybutyrate) depolymerase -
MKLM6_RS22115 22045..22542 - 498 Protein_26 hypothetical protein -
MKLM6_RS22120 22693..23019 - 327 WP_054759895.1 EthD family reductase -
MKLM6_RS22125 23137..23286 + 150 Protein_28 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..186658 186658


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-110)

Antitoxin

Antitoxin PemI-like

(1-79)


Sequences

Toxin        


Download         Length: 113 a.a.        Molecular weight: 12138.09 Da        Isoelectric Point: 5.8269

>T85861 WP_096876826.1 NZ_CP023670:c19268-18930 [Methylomonas koyamae]
MVVSYVPERGDLVWIAFDPQAGHEQAGRRPGLVVTPRTYNVKAGLALICPVTSRVKGYPFEVPLPDGDRVSGVILADQIR
SLDWAARQAEKISMVDGAVLAEVLAKIEALIQ

Download         Length: 339 bp

>T85861 NZ_CP023670:c19268-18930 [Methylomonas koyamae]
ATGGTTGTATCGTACGTACCTGAGCGCGGGGATTTGGTGTGGATTGCGTTCGATCCTCAAGCAGGCCACGAACAAGCGGG
GCGCCGCCCCGGCCTGGTCGTTACGCCACGCACTTATAACGTCAAAGCCGGGTTGGCGTTGATTTGCCCTGTAACAAGTC
GGGTTAAAGGATATCCGTTTGAGGTACCGTTACCGGACGGCGACCGTGTGAGTGGTGTGATATTGGCGGATCAGATTCGG
AGTCTGGATTGGGCGGCGCGTCAGGCCGAAAAGATATCTATGGTGGACGGGGCGGTATTGGCCGAAGTCTTGGCCAAAAT
CGAGGCTCTTATTCAATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8911.24 Da        Isoelectric Point: 4.7204

>AT85861 WP_096876827.1 NZ_CP023670:c19516-19268 [Methylomonas koyamae]
MQTEIKKWGNSAVIRLPAAMLAQLKLEVGSPVELTADDAGLRIVPTTAKPVFKLEELLAGMTPDNRHDCIDWGPDVGREG
NN

Download         Length: 249 bp

>AT85861 NZ_CP023670:c19516-19268 [Methylomonas koyamae]
ATGCAAACTGAAATTAAAAAATGGGGTAATAGCGCCGTGATTCGATTGCCGGCGGCGATGCTGGCACAGTTAAAGCTGGA
AGTCGGTTCGCCGGTCGAGCTGACAGCGGACGATGCCGGGCTACGGATTGTACCGACCACGGCCAAACCTGTTTTTAAGT
TGGAGGAATTGCTGGCTGGCATGACTCCCGATAATCGGCATGATTGCATTGACTGGGGGCCGGATGTTGGCCGAGAAGGT
AACAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References