Detailed information of TA system TA085860

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3959937..3960578
• Replicon: chromosome
• Accession: NZ_CP023669
• Organism: Methylomonas koyamae strain LM6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MKLM6_RS17755
• Protein ID: WP_096876423.1
• Coordinates: 3960177..3960578 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A291IN90
• Locus tag: MKLM6_RS17750
• Protein ID: WP_054761998.1
• Coordinates: 3959937..3960170 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKLM6_RS17725	3955399..3957216	-	1818	WP_096876792.1	ABC transporter ATP-binding protein/permease	-
MKLM6_RS17730	3957656..3957910	+	255	WP_096876421.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
MKLM6_RS17735	3957907..3958320	+	414	WP_064026136.1	type II toxin-antitoxin system VapC family toxin	-
MKLM6_RS17740	3958925..3959191	+	267	WP_064026134.1	DUF2281 domain-containing protein	-
MKLM6_RS17745	3959191..3959574	+	384	WP_096876422.1	type II toxin-antitoxin system VapC family toxin	-
MKLM6_RS17750	3959937..3960170	+	234	WP_054761998.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MKLM6_RS17755	3960177..3960578	+	402	WP_096876423.1	type II toxin-antitoxin system VapC family toxin	Toxin
MKLM6_RS17760	3961303..3963102	+	1800	WP_096876424.1	DUF262 domain-containing protein	-
MKLM6_RS17770	3963807..3964214	-	408	WP_096876425.1	type II toxin-antitoxin system VapC family toxin	-
MKLM6_RS17775	3964219..3964476	-	258	WP_054762008.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-116)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14882.27 Da
• Isoelectric Point: 7.1285

>T85860 WP_096876423.1 NZ_CP023669:3960177-3960578 [Methylomonas koyamae]
MLKYLLDTNIVIYVIKQRPIEVLATFNRHHGRMAISAITLAELIHGAEKSQFPARNLATVEDFCSRLQVLPYTDSSAMHY
GGIRAALEKIGQPIGVNDLHIAAHARSHGLILVSNNLPEFERVPGLLMENWLE

Nucleotide

• Length: 402 bp

>T85860 NZ_CP023669:3960177-3960578 [Methylomonas koyamae]
ATGTTGAAATATCTGCTCGACACCAACATCGTCATCTATGTGATCAAACAACGGCCGATCGAAGTATTGGCGACCTTCAA
CCGGCATCACGGCCGCATGGCGATTTCCGCGATTACGCTGGCTGAACTGATCCACGGCGCCGAAAAGAGCCAATTCCCCG
CCCGCAACCTTGCTACGGTGGAAGACTTTTGCAGCCGTTTACAAGTATTGCCTTACACCGACAGCTCCGCGATGCATTAC
GGCGGCATACGCGCCGCGTTGGAGAAAATCGGCCAGCCGATAGGTGTCAACGACCTGCACATCGCCGCTCATGCCCGCAG
CCACGGCTTGATTTTGGTCAGCAATAATCTGCCAGAATTCGAGCGGGTGCCGGGGTTGTTGATGGAGAATTGGTTGGAAT
GA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8618.53 Da
• Isoelectric Point: 4.4883

>AT85860 WP_054761998.1 NZ_CP023669:3959937-3960170 [Methylomonas koyamae]
MATSTVFTNNRSQAVRLPADMRFPDSVKKVEVRAVGQERIIAPADAAWDSFFLDGPSVSDDFLNERPSQQQGERESF

Nucleotide

• Length: 234 bp

>AT85860 NZ_CP023669:3959937-3960170 [Methylomonas koyamae]
ATGGCGACCAGTACCGTATTTACCAACAACCGCAGTCAGGCCGTGCGCCTACCCGCCGACATGCGCTTTCCCGACAGCGT
CAAAAAGGTTGAGGTTCGTGCCGTCGGGCAGGAACGCATCATTGCCCCGGCGGACGCGGCGTGGGACAGCTTTTTTCTCG
ATGGCCCGAGCGTCAGCGACGATTTTTTAAACGAACGCCCGAGCCAGCAGCAAGGCGAGCGCGAGAGTTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A291IN90