Detailed information of TA system TA085859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3957656..3958320
• Replicon: chromosome
• Accession: NZ_CP023669
• Organism: Methylomonas koyamae strain LM6

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: A0A291IMQ7
• Locus tag: MKLM6_RS17735
• Protein ID: WP_064026136.1
• Coordinates: 3957907..3958320 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: -
• Locus tag: MKLM6_RS17730
• Protein ID: WP_096876421.1
• Coordinates: 3957656..3957910 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKLM6_RS17720	3954200..3955153	-	954	WP_064026141.1	hypothetical protein	-
MKLM6_RS17725	3955399..3957216	-	1818	WP_096876792.1	ABC transporter ATP-binding protein/permease	-
MKLM6_RS17730	3957656..3957910	+	255	WP_096876421.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MKLM6_RS17735	3957907..3958320	+	414	WP_064026136.1	type II toxin-antitoxin system VapC family toxin	Toxin
MKLM6_RS17740	3958925..3959191	+	267	WP_064026134.1	DUF2281 domain-containing protein	-
MKLM6_RS17745	3959191..3959574	+	384	WP_096876422.1	type II toxin-antitoxin system VapC family toxin	-
MKLM6_RS17750	3959937..3960170	+	234	WP_054761998.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
MKLM6_RS17755	3960177..3960578	+	402	WP_096876423.1	type II toxin-antitoxin system VapC family toxin	-
MKLM6_RS17760	3961303..3963102	+	1800	WP_096876424.1	DUF262 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

• SpoVT-AbrB domain (9-46)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15329.83 Da
• Isoelectric Point: 6.7055

>T85859 WP_064026136.1 NZ_CP023669:3957907-3958320 [Methylomonas koyamae]
MIYMLDTNMLIYLIKNKPPSVAERINNLPENSALCMSFVTYAELLKGAEGSLRKEKVLAQLEQLLRIVPVEYAVGGALCR
HYASQFTRLKTAGTPIGANDLWIACHALAIGARLVTHNVREFERIEGLAVEDWADER

Nucleotide

• Length: 414 bp

>T85859 NZ_CP023669:3957907-3958320 [Methylomonas koyamae]
ATGATCTATATGTTGGATACCAACATGCTGATCTATCTGATCAAAAACAAGCCGCCTTCTGTAGCCGAACGCATCAACAA
CCTCCCGGAAAATAGCGCGCTGTGCATGTCGTTCGTCACTTATGCCGAGTTGTTGAAGGGTGCTGAGGGCAGCCTACGCA
AGGAAAAAGTGTTGGCGCAGTTGGAACAATTGCTGCGGATCGTACCGGTCGAATATGCTGTTGGCGGTGCACTATGCCGA
CACTACGCCAGCCAATTTACCCGGCTAAAAACGGCCGGAACGCCGATAGGCGCCAACGATTTGTGGATTGCCTGCCATGC
GCTGGCGATTGGCGCCCGCTTGGTCACGCATAACGTGCGGGAATTCGAGCGAATTGAGGGGTTGGCCGTCGAGGATTGGG
CGGATGAGCGGTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9499.77 Da
• Isoelectric Point: 4.7769

>AT85859 WP_096876421.1 NZ_CP023669:3957656-3957910 [Methylomonas koyamae]
MRTLTTRIFKNGNSQAVRIPQEFRLEVSQVEISRCPNGDLLIHPVPADRGAALLAVLQGFDDDFAEHLLENAREQVPMQE
RESL

Nucleotide

• Length: 255 bp

>AT85859 NZ_CP023669:3957656-3957910 [Methylomonas koyamae]
ATGAGGACTTTGACTACCCGCATTTTCAAAAACGGCAACAGCCAAGCAGTTAGAATTCCGCAGGAATTTCGGCTGGAGGT
TAGCCAAGTGGAAATCAGTCGTTGCCCCAACGGCGATTTGCTGATTCATCCGGTACCGGCCGACCGGGGCGCGGCGTTGC
TGGCGGTTTTGCAAGGTTTCGACGACGACTTTGCCGAACATTTGCTCGAAAACGCCCGCGAGCAAGTCCCGATGCAGGAG
CGCGAGTCTTTATGA

Structures

Toxin

• AlphaFold DB: A0A291IMQ7