Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 2567313..2568805 Replicon chromosome
Accession NZ_CP023669
Organism Methylomonas koyamae strain LM6

Toxin (Protein)


Gene name HipA1 Uniprot ID A0A291IK14
Locus tag MKLM6_RS11520 Protein ID WP_064029880.1
Coordinates 2567543..2568805 (+) Length 421 a.a.

Antitoxin (Protein)


Gene name HipB1 Uniprot ID A0A291IJW7
Locus tag MKLM6_RS11515 Protein ID WP_064029878.1
Coordinates 2567313..2567546 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKLM6_RS11485 2562685..2563047 + 363 WP_157770297.1 hypothetical protein -
MKLM6_RS11490 2563037..2564749 + 1713 WP_096876119.1 copper resistance system multicopper oxidase -
MKLM6_RS11495 2564823..2565428 + 606 WP_096876752.1 copper resistance protein B -
MKLM6_RS11500 2565530..2566009 + 480 WP_198402711.1 aspartate carbamoyltransferase -
MKLM6_RS23490 2566514..2567065 - 552 WP_064029877.1 hypothetical protein -
MKLM6_RS11515 2567313..2567546 + 234 WP_064029878.1 helix-turn-helix transcriptional regulator Antitoxin
MKLM6_RS11520 2567543..2568805 + 1263 WP_064029880.1 type II toxin-antitoxin system HipA family toxin Toxin
MKLM6_RS11525 2569199..2570446 - 1248 WP_096875497.1 IS256 family transposase -
MKLM6_RS11530 2570519..2570731 - 213 WP_096876121.1 hypothetical protein -
MKLM6_RS11535 2570766..2570975 - 210 WP_064029884.1 DUF3820 family protein -
MKLM6_RS11540 2570975..2571238 - 264 WP_064029885.1 hypothetical protein -
MKLM6_RS23495 2571247..2571566 - 320 Protein_2280 hypothetical protein -
MKLM6_RS11550 2571582..2572352 - 771 WP_064029893.1 zinc-dependent peptidase -
MKLM6_RS23500 2572477..2572653 - 177 WP_197485159.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(147-376)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-62)


Sequences

Toxin        


Download         Length: 421 a.a.        Molecular weight: 46409.24 Da        Isoelectric Point: 6.6039

>T85857 WP_064029880.1 NZ_CP023669:2567543-2568805 [Methylomonas koyamae]
MSHLLAVYLFDRRVGDLALVEGRLQFSYVADWLQQADAAPLSCSLPLQVEPFNDQQTRPFFAGLLPEGKLRQLIARQFQI
SRQNDFALLDHIGGECAGAVSLLQPDQTPLVPKTTNEIDWLSNDDVVALLDELPQRPMLAGQDGLRLSLAGAQDKLPVVF
DGQRIGLPRNGTPSSHILKPAIAAVEDSVINEAFCMTLARAMQLQTAQSQIGSIGERQFLLVERYDRTLAANGQRMRLHQ
EDFCQALGVVPELKYQNEGGPGLEQCFDLVRRVTRPSAPQVLRLLDAVIFNALIGNHDAHAKNFSILYADKGAVLAPLYD
VLSTAVYPSLTPKMAMQLGGKYKFSEVQARHWDRFAQSAGLSVAQTRKRILGLCQRLPLVARTLQASADYEFTGNSVVEQ
IVQLIEQRAALTVRRLTDTA

Download         Length: 1263 bp

>T85857 NZ_CP023669:2567543-2568805 [Methylomonas koyamae]
ATGAGTCATTTGCTTGCCGTCTATTTATTTGATCGTCGAGTGGGGGATTTGGCCCTGGTTGAAGGCAGACTGCAATTTAG
CTACGTAGCCGACTGGCTGCAGCAAGCCGACGCTGCCCCGTTGTCCTGTTCGTTACCGTTGCAAGTTGAGCCGTTCAACG
ACCAACAGACACGACCGTTCTTTGCCGGCCTGTTACCGGAAGGCAAATTACGCCAATTGATCGCTCGCCAGTTTCAGATC
TCGAGACAAAATGACTTTGCCTTGCTCGATCATATCGGCGGCGAATGCGCCGGTGCCGTTTCACTGCTACAGCCTGACCA
AACACCGCTTGTACCGAAAACGACGAATGAGATTGACTGGTTATCCAACGATGATGTAGTTGCCTTGCTGGATGAATTGC
CGCAGCGGCCGATGCTGGCCGGCCAGGATGGTTTGCGTTTATCACTGGCGGGCGCTCAGGATAAATTGCCGGTGGTGTTT
GATGGTCAACGGATCGGTCTGCCCCGAAACGGCACGCCCAGTAGCCATATTCTCAAACCGGCCATTGCTGCCGTTGAAGA
CAGTGTGATCAACGAAGCTTTTTGCATGACATTGGCCAGAGCAATGCAACTTCAGACCGCACAGTCGCAAATTGGCAGCA
TTGGTGAGCGCCAATTTCTGTTGGTTGAGCGTTACGACAGAACACTGGCTGCAAATGGCCAACGCATGCGACTGCATCAG
GAAGATTTCTGCCAGGCACTTGGCGTGGTGCCCGAATTGAAATACCAGAACGAGGGTGGGCCGGGTTTGGAACAATGCTT
TGACTTGGTCCGCCGCGTGACCCGACCGAGTGCGCCGCAGGTGTTGCGTCTGCTGGATGCGGTCATTTTCAATGCCTTGA
TCGGCAACCACGACGCCCATGCCAAGAACTTTTCTATTTTGTATGCCGACAAGGGGGCCGTTCTCGCGCCGCTCTACGAC
GTATTGTCAACCGCCGTTTATCCCAGTCTGACGCCCAAGATGGCGATGCAGTTGGGCGGCAAATACAAATTCAGCGAAGT
GCAGGCCCGGCATTGGGATCGTTTTGCCCAGTCAGCGGGCTTATCGGTAGCGCAAACCCGAAAACGCATCCTCGGCTTGT
GCCAACGGCTACCGCTGGTCGCGCGCACTTTGCAGGCATCGGCGGATTATGAATTCACTGGAAATTCGGTGGTTGAACAA
ATCGTACAGTTGATCGAGCAACGCGCGGCGCTGACGGTCAGGCGATTGACTGATACGGCCTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8169.55 Da        Isoelectric Point: 4.9009

>AT85857 WP_064029878.1 NZ_CP023669:2567313-2567546 [Methylomonas koyamae]
MINIQSTQQLGQALRTARKQLGLTQSELALAAGVGVRFIVDLEAGKPTVRLETVMRVIEALGGQVMLDGLPDIAEAP

Download         Length: 234 bp

>AT85857 NZ_CP023669:2567313-2567546 [Methylomonas koyamae]
ATGATCAACATCCAATCCACCCAACAACTTGGCCAAGCGCTACGCACCGCTCGCAAACAGCTCGGATTAACCCAGTCCGA
ACTGGCGTTGGCGGCAGGCGTGGGTGTTCGCTTTATTGTTGACCTCGAAGCCGGCAAGCCGACCGTGCGCCTGGAAACGG
TGATGCGCGTGATCGAGGCATTGGGAGGTCAGGTCATGCTCGATGGCCTGCCGGACATCGCCGAAGCACCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A291IK14

Antitoxin

Source ID Structure
AlphaFold DB A0A291IJW7

References