Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1629439..1630076 Replicon chromosome
Accession NZ_CP023669
Organism Methylomonas koyamae strain LM6

Toxin (Protein)


Gene name vapC Uniprot ID A0A291IHT2
Locus tag MKLM6_RS07680 Protein ID WP_064028068.1
Coordinates 1629439..1629846 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name VapB1 Uniprot ID A0A291IHH1
Locus tag MKLM6_RS07685 Protein ID WP_064028070.1
Coordinates 1629846..1630076 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKLM6_RS07655 1624525..1625139 + 615 WP_064028061.1 DUF938 domain-containing protein -
MKLM6_RS07660 1625136..1625642 + 507 WP_064028063.1 M48 family metallopeptidase -
MKLM6_RS07665 1626130..1627542 - 1413 WP_064028065.1 hypothetical protein -
MKLM6_RS07670 1627535..1628839 - 1305 WP_064028067.1 ParA family protein -
MKLM6_RS07675 1628836..1629252 - 417 WP_054762545.1 hypothetical protein -
MKLM6_RS07680 1629439..1629846 - 408 WP_064028068.1 type II toxin-antitoxin system VapC family toxin Toxin
MKLM6_RS07685 1629846..1630076 - 231 WP_064028070.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
MKLM6_RS07690 1630309..1630899 - 591 WP_064028072.1 Uma2 family endonuclease -
MKLM6_RS07695 1631251..1631625 - 375 WP_197485061.1 DUF86 domain-containing protein -
MKLM6_RS07700 1631606..1631986 - 381 WP_096875867.1 nucleotidyltransferase domain-containing protein -
MKLM6_RS07705 1632002..1634818 - 2817 WP_064028076.1 excinuclease ABC subunit UvrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-126)

Antitoxin

SpoVT-AbrB domain

(7-43)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15318.47 Da        Isoelectric Point: 6.2340

>T85856 WP_064028068.1 NZ_CP023669:c1629846-1629439 [Methylomonas koyamae]
MASRYLLDTNICIYIAKYRPPAVRERFARHSASELAMSVITLGELRFGAEKSQARDRAITAIDELTGLIPICDLPETAGE
HYGQIRAELQKIGQPIGNNDLWLAAHARSQEWVLVTNNEREFLRVPDLRVENWVS

Download         Length: 408 bp

>T85856 NZ_CP023669:c1629846-1629439 [Methylomonas koyamae]
ATGGCGTCGCGTTACCTGCTGGACACCAACATCTGCATCTACATTGCAAAATACCGCCCTCCCGCCGTGCGCGAGCGCTT
CGCCCGCCACTCAGCCAGCGAACTGGCTATGTCGGTTATTACCTTGGGCGAATTGCGCTTCGGCGCCGAAAAAAGCCAGG
CGCGGGACCGAGCTATTACAGCCATTGACGAATTAACCGGCCTGATTCCGATCTGCGACTTGCCCGAAACGGCCGGGGAA
CATTACGGACAAATCCGCGCGGAGCTACAAAAAATCGGCCAACCGATAGGCAATAACGATTTATGGCTGGCCGCCCATGC
TCGGTCTCAAGAGTGGGTATTGGTGACCAACAATGAACGCGAATTTCTCAGAGTGCCGGATTTGCGGGTTGAGAATTGGG
TGAGTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8711.66 Da        Isoelectric Point: 4.3449

>AT85856 WP_064028070.1 NZ_CP023669:c1630076-1629846 [Methylomonas koyamae]
MTYARIFQSGNSQAVRLPKEFRFNADRVEIFRRGDEIVLRETPTNATAIFDALSALPEDFMAEGREDDAPQEREGF

Download         Length: 231 bp

>AT85856 NZ_CP023669:c1630076-1629846 [Methylomonas koyamae]
ATGACCTACGCCCGTATTTTTCAATCCGGCAACAGCCAAGCCGTGCGCTTGCCCAAGGAATTTAGGTTTAACGCCGACCG
TGTGGAGATCTTTCGCCGCGGCGACGAAATCGTGTTACGAGAAACCCCGACCAACGCCACGGCAATATTCGACGCTCTCA
GCGCCTTACCGGAAGATTTCATGGCAGAAGGCCGCGAAGACGATGCACCGCAAGAACGCGAAGGGTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A291IHT2

Antitoxin

Source ID Structure
AlphaFold DB A0A291IHH1

References