Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-yefM/YoeB-YefM
Location 1428601..1429109 Replicon chromosome
Accession NZ_CP023669
Organism Methylomonas koyamae strain LM6

Toxin (Protein)


Gene name relE Uniprot ID A0A291IHD0
Locus tag MKLM6_RS06730 Protein ID WP_064028910.1
Coordinates 1428849..1429109 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A172U6C3
Locus tag MKLM6_RS06725 Protein ID WP_054761862.1
Coordinates 1428601..1428852 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKLM6_RS06700 1424021..1424452 - 432 WP_096875835.1 urease accessory protein UreE -
MKLM6_RS06705 1424686..1426389 - 1704 WP_096875836.1 urease subunit alpha -
MKLM6_RS06710 1426386..1426703 - 318 WP_096875837.1 urease subunit beta -
MKLM6_RS06715 1427010..1428047 + 1038 WP_064024692.1 IS110 family transposase -
MKLM6_RS06720 1428159..1428461 - 303 WP_054761860.1 urease subunit gamma -
MKLM6_RS06725 1428601..1428852 + 252 WP_054761862.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
MKLM6_RS06730 1428849..1429109 + 261 WP_064028910.1 Txe/YoeB family addiction module toxin Toxin
MKLM6_RS06735 1429578..1430741 - 1164 WP_064028912.1 hypothetical protein -
MKLM6_RS06745 1431402..1431668 - 267 WP_082889573.1 type II toxin-antitoxin system RelE/ParE family toxin -
MKLM6_RS06750 1431668..1431892 - 225 WP_198402764.1 hypothetical protein -
MKLM6_RS06755 1431926..1432777 - 852 WP_064028917.1 urease accessory protein UreD -
MKLM6_RS06760 1432790..1433095 - 306 WP_064028919.1 hypothetical protein -
MKLM6_RS06765 1433189..1433962 - 774 WP_064028921.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 10224.71 Da        Isoelectric Point: 9.6408

>T85855 WP_064028910.1 NZ_CP023669:1428849-1429109 [Methylomonas koyamae]
VKITFSSDAWDSYLYWQATDKAILKRINQLIKEIQRQPFEGIGKPEPLKHGLSGYWSRRINDEHRIVYKVAGDTLLIAQL
RFHYEY

Download         Length: 261 bp

>T85855 NZ_CP023669:1428849-1429109 [Methylomonas koyamae]
GTGAAAATCACCTTTTCGTCCGACGCCTGGGACAGTTACCTGTACTGGCAAGCCACCGACAAAGCCATACTTAAACGCAT
CAACCAACTGATCAAGGAAATCCAGCGCCAACCGTTCGAGGGCATCGGCAAGCCGGAACCGTTGAAGCACGGTTTGTCCG
GCTACTGGTCGCGGCGGATTAACGACGAGCATAGGATCGTTTACAAGGTTGCCGGCGATACGTTGTTGATTGCGCAGTTG
CGGTTTCACTATGAGTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9170.43 Da        Isoelectric Point: 4.4621

>AT85855 WP_054761862.1 NZ_CP023669:1428601-1428852 [Methylomonas koyamae]
MDAISYSAARANLAKTMDKVCADHAPIIITRKSESPVVMLSLEDYQAMEETAYLLRSPANARRLLESIAELESGQGSERE
LIE

Download         Length: 252 bp

>AT85855 NZ_CP023669:1428601-1428852 [Methylomonas koyamae]
ATGGACGCCATCAGTTACAGCGCCGCTCGCGCCAATTTAGCCAAAACCATGGACAAGGTTTGCGCCGACCATGCCCCCAT
CATCATCACCCGCAAAAGCGAGTCGCCGGTGGTGATGCTGTCGCTGGAAGACTACCAAGCCATGGAAGAAACCGCCTACC
TGCTGCGCTCGCCAGCCAACGCCCGCCGCTTGCTGGAATCCATTGCCGAATTGGAATCCGGCCAAGGCAGCGAGCGCGAG
TTGATCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A291IHD0

Antitoxin

Source ID Structure
AlphaFold DB A0A172U6C3

References