Detailed information of TA system TA085850

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 913967..914886
• Replicon: chromosome
• Accession: NZ_CP023666
• Organism: Bacillus paralicheniformis strain Bac48

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A6I7TYV7
• Locus tag: CP942_RS04520
• Protein ID: WP_023856735.1
• Coordinates: 913967..914713 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: A0A6I7TM13
• Locus tag: CP942_RS04525
• Protein ID: WP_023856734.1
• Coordinates: 914713..914886 (+)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP942_RS04500	909360..910004	-	645	WP_026579842.1	YesL family protein	-
CP942_RS04505	910139..911896	-	1758	WP_026579843.1	gamma-glutamyltransferase	-
CP942_RS04510	912229..912846	+	618	WP_023856737.1	DUF47 domain-containing protein	-
CP942_RS04515	912859..913857	+	999	WP_075212749.1	inorganic phosphate transporter	-
CP942_RS04520	913967..914713	+	747	WP_023856735.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
CP942_RS04525	914713..914886	+	174	WP_023856734.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
CP942_RS04530	915178..916449	-	1272	WP_026579844.1	MFS transporter	-
CP942_RS04535	916501..917514	-	1014	WP_079289562.1	zinc-binding alcohol dehydrogenase family protein	-
CP942_RS04540	917540..918388	-	849	WP_023856787.1	SDR family oxidoreductase	-
CP942_RS04545	918364..919461	-	1098	WP_025811596.1	mannonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-57)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29421.87 Da
• Isoelectric Point: 4.6015

>T85850 WP_023856735.1 NZ_CP023666:913967-914713 [Bacillus paralicheniformis]
MLLFYQFLVWLIVLALALYVAAVWRFEKKLTEKTVAIRKTWYLLYVIGAVIYWTHDPQSIFTNPLHYLIVAVFFTLTDAF
IFLNAYFKKLGSSELATDTRMLLEENNDLLHTYQNRLKTFQYLLKNEPIHIYYGNIEAYAEGIEKLIKRFAEKMNISAAL
CEYNSEESKDHLLEHMENRFDVQEKLERKDVYYEENGKMVLIPFSIHDFDYVMKLTSEDLVTEFDYLLFTSLTSIYDLLL
PNEEDGDD

Nucleotide

• Length: 747 bp

>T85850 NZ_CP023666:913967-914713 [Bacillus paralicheniformis]
ATGCTGCTATTTTATCAATTCCTTGTTTGGCTCATTGTATTGGCGCTGGCTCTGTATGTGGCTGCCGTGTGGCGTTTTGA
AAAAAAGCTCACCGAAAAAACGGTCGCGATCCGAAAAACTTGGTACCTGCTTTATGTTATCGGCGCCGTGATTTATTGGA
CGCACGATCCGCAGTCGATTTTCACAAACCCGCTTCATTACTTGATCGTTGCCGTTTTTTTCACGCTGACAGACGCTTTT
ATTTTCTTAAACGCTTACTTTAAAAAGCTCGGCAGCTCCGAACTCGCGACAGACACAAGAATGCTTCTCGAAGAAAACAA
CGACCTCCTCCACACATATCAAAACAGGCTGAAAACGTTTCAATACCTATTGAAAAACGAACCGATCCACATCTATTATG
GAAATATAGAAGCATATGCAGAAGGGATCGAAAAGCTCATCAAACGGTTTGCCGAAAAAATGAATATATCCGCTGCACTT
TGCGAATATAATTCAGAAGAAAGCAAGGATCATTTGCTTGAGCATATGGAAAACCGCTTCGATGTTCAAGAAAAGCTTGA
GCGCAAAGACGTTTATTATGAAGAAAACGGAAAAATGGTCCTCATTCCTTTTTCCATCCACGATTTTGACTATGTCATGA
AGTTAACCTCAGAAGACCTTGTCACAGAATTTGACTACCTCCTGTTTACTTCTTTAACAAGCATCTATGATCTTCTGCTA
CCAAACGAAGAGGACGGTGACGACTGA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6897.99 Da
• Isoelectric Point: 10.8356

>AT85850 WP_023856734.1 NZ_CP023666:914713-914886 [Bacillus paralicheniformis]
MEPAFQQDHESAKKRLAFKMFKKQSKTNIAKYEVSPHTERIFRQNERLIGEYKRKKA

Nucleotide

• Length: 174 bp

>AT85850 NZ_CP023666:914713-914886 [Bacillus paralicheniformis]
ATGGAGCCTGCTTTTCAGCAAGACCATGAAAGCGCAAAAAAGCGCCTCGCCTTTAAAATGTTTAAAAAGCAATCCAAAAC
CAATATCGCGAAATACGAAGTCAGCCCCCATACCGAACGGATTTTCCGGCAAAACGAACGGCTGATCGGCGAGTATAAAA
GAAAAAAAGCTTAA

Structures

Toxin

• AlphaFold DB: A0A6I7TYV7

Antitoxin

• AlphaFold DB: A0A6I7TM13