Detailed information of TA system TA085849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1453766..1454685
• Replicon: chromosome
• Accession: NZ_CP023665
• Organism: Bacillus paralicheniformis strain Bac84

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A6I7TYV7
• Locus tag: CP943_RS07480
• Protein ID: WP_023856735.1
• Coordinates: 1453939..1454685 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: A0A6I7TM13
• Locus tag: CP943_RS07475
• Protein ID: WP_023856734.1
• Coordinates: 1453766..1453939 (-)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP943_RS07455	1449191..1450288	+	1098	WP_105980895.1	mannonate dehydratase	-
CP943_RS07460	1450264..1451112	+	849	WP_105981356.1	SDR family oxidoreductase	-
CP943_RS07465	1451138..1452151	+	1014	WP_105980896.1	zinc-binding alcohol dehydrogenase family protein	-
CP943_RS07470	1452203..1453474	+	1272	WP_023856732.1	MFS transporter	-
CP943_RS07475	1453766..1453939	-	174	WP_023856734.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
CP943_RS07480	1453939..1454685	-	747	WP_023856735.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
CP943_RS07485	1454795..1455793	-	999	WP_075212749.1	inorganic phosphate transporter	-
CP943_RS07490	1455806..1456423	-	618	WP_023856737.1	DUF47 domain-containing protein	-
CP943_RS07495	1456756..1458513	+	1758	WP_026579843.1	gamma-glutamyltransferase	-
CP943_RS07500	1458648..1459292	+	645	WP_026579842.1	YesL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-57)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29421.87 Da
• Isoelectric Point: 4.6015

>T85849 WP_023856735.1 NZ_CP023665:c1454685-1453939 [Bacillus paralicheniformis]
MLLFYQFLVWLIVLALALYVAAVWRFEKKLTEKTVAIRKTWYLLYVIGAVIYWTHDPQSIFTNPLHYLIVAVFFTLTDAF
IFLNAYFKKLGSSELATDTRMLLEENNDLLHTYQNRLKTFQYLLKNEPIHIYYGNIEAYAEGIEKLIKRFAEKMNISAAL
CEYNSEESKDHLLEHMENRFDVQEKLERKDVYYEENGKMVLIPFSIHDFDYVMKLTSEDLVTEFDYLLFTSLTSIYDLLL
PNEEDGDD

Nucleotide

• Length: 747 bp

>T85849 NZ_CP023665:c1454685-1453939 [Bacillus paralicheniformis]
ATGCTGCTATTTTATCAATTCCTTGTTTGGCTCATTGTATTGGCGCTGGCTCTGTATGTGGCTGCCGTGTGGCGTTTTGA
AAAAAAGCTCACCGAAAAAACGGTCGCGATCCGAAAAACTTGGTACCTGCTTTATGTTATCGGCGCCGTGATTTATTGGA
CGCACGATCCGCAGTCGATTTTCACAAACCCGCTTCATTACCTGATCGTTGCCGTTTTTTTCACGCTGACAGACGCTTTT
ATTTTCTTAAACGCTTACTTTAAAAAGCTCGGCAGCTCCGAACTCGCGACAGACACAAGAATGCTTCTCGAAGAAAACAA
CGACCTCCTCCACACATATCAAAACAGGCTGAAAACGTTTCAATACCTATTGAAAAACGAACCGATCCACATCTATTATG
GAAATATAGAAGCATATGCAGAAGGGATCGAAAAGCTCATCAAACGATTTGCCGAAAAAATGAATATATCCGCTGCACTT
TGCGAATATAATTCAGAAGAAAGCAAGGATCATTTGCTTGAGCATATGGAAAACCGCTTCGATGTTCAAGAAAAGCTTGA
GCGCAAAGACGTTTATTATGAAGAAAACGGAAAAATGGTCCTCATTCCTTTTTCCATCCACGATTTTGACTATGTCATGA
AGTTAACCTCAGAAGACCTTGTCACAGAATTTGACTACCTCCTGTTTACTTCTTTAACAAGCATCTATGATCTTCTGCTA
CCAAACGAAGAGGACGGTGACGACTGA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6897.99 Da
• Isoelectric Point: 10.8356

>AT85849 WP_023856734.1 NZ_CP023665:c1453939-1453766 [Bacillus paralicheniformis]
MEPAFQQDHESAKKRLAFKMFKKQSKTNIAKYEVSPHTERIFRQNERLIGEYKRKKA

Nucleotide

• Length: 174 bp

>AT85849 NZ_CP023665:c1453939-1453766 [Bacillus paralicheniformis]
ATGGAGCCTGCTTTTCAGCAAGACCATGAAAGCGCAAAAAAGCGCCTCGCCTTTAAAATGTTTAAAAAGCAATCCAAAAC
CAATATCGCGAAATACGAAGTCAGCCCCCATACCGAACGGATTTTCCGGCAAAACGAACGGCTGATCGGCGAGTATAAAA
GAAAAAAAGCTTAA

Structures

Toxin

• AlphaFold DB: A0A6I7TYV7

Antitoxin

• AlphaFold DB: A0A6I7TM13