Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 616472..617108 Replicon chromosome
Accession NZ_CP023665
Organism Bacillus paralicheniformis strain Bac84

Toxin (Protein)


Gene name mazF Uniprot ID M5P3Q9
Locus tag CP943_RS03145 Protein ID WP_003179128.1
Coordinates 616758..617108 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M5PDU2
Locus tag CP943_RS03140 Protein ID WP_006638778.1
Coordinates 616472..616753 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP943_RS03120 611583..613067 + 1485 WP_039073238.1 PH domain-containing protein -
CP943_RS03125 613064..613663 - 600 WP_023857867.1 rhomboid family intramembrane serine protease -
CP943_RS03130 614007..614966 + 960 WP_164468292.1 outer membrane lipoprotein carrier protein LolA -
CP943_RS03135 615191..616360 + 1170 WP_026580588.1 alanine racemase -
CP943_RS03140 616472..616753 + 282 WP_006638778.1 hypothetical protein Antitoxin
CP943_RS03145 616758..617108 + 351 WP_003179128.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CP943_RS03150 617226..618053 + 828 WP_025810067.1 RsbT co-antagonist protein RsbRA -
CP943_RS03155 618057..618422 + 366 WP_020450259.1 STAS domain-containing protein -
CP943_RS03160 618425..618826 + 402 WP_020450260.1 anti-sigma regulatory factor -
CP943_RS03165 618837..619844 + 1008 WP_105980729.1 PP2C family protein-serine/threonine phosphatase -
CP943_RS03170 619903..620229 + 327 WP_020450262.1 anti-sigma factor antagonist -
CP943_RS03175 620229..620711 + 483 WP_020450263.1 anti-sigma B factor RsbW -
CP943_RS03180 620677..621468 + 792 WP_023857858.1 RNA polymerase sigma factor SigB -
CP943_RS03185 621465..622064 + 600 WP_023857857.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12990.03 Da        Isoelectric Point: 5.7234

>T85848 WP_003179128.1 NZ_CP023665:616758-617108 [Bacillus paralicheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Download         Length: 351 bp

>T85848 NZ_CP023665:616758-617108 [Bacillus paralicheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGATTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10649.03 Da        Isoelectric Point: 4.6920

>AT85848 WP_006638778.1 NZ_CP023665:616472-616753 [Bacillus paralicheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Download         Length: 282 bp

>AT85848 NZ_CP023665:616472-616753 [Bacillus paralicheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTGCGCGAGCGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATTAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I7FHI4

Antitoxin

Source ID Structure
AlphaFold DB M5PDU2

References