Detailed information of TA system TA085831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 92002..92579
• Replicon: plasmid pApBDGP5A
• Accession: NZ_CP023658
• Organism: Acetobacter pomorum strain BDGP5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C7JIL1
• Locus tag: CPF11_RS13820
• Protein ID: WP_006115553.1
• Coordinates: 92271..92579 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C7JIL2
• Locus tag: CPF11_RS13815
• Protein ID: WP_006115552.1
• Coordinates: 92002..92274 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPF11_RS13785	87625..87774	-	150	WP_007397858.1	DUF2474 domain-containing protein	-
CPF11_RS13790	87761..88780	-	1020	WP_006115550.1	cytochrome d ubiquinol oxidase subunit II	-
CPF11_RS13795	88783..90216	-	1434	WP_006115551.1	cytochrome ubiquinol oxidase subunit I	-
CPF11_RS13805	90652..91611	-	960	WP_035350857.1	IS481 family transposase	-
CPF11_RS13810	91669..91929	+	261	Protein_79	hypothetical protein	-
CPF11_RS13815	92002..92274	+	273	WP_006115552.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CPF11_RS13820	92271..92579	+	309	WP_006115553.1	PIN domain-containing protein	Toxin
CPF11_RS13825	92678..92932	+	255	WP_006115554.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
CPF11_RS13830	92934..93263	+	330	WP_012813213.1	endoribonuclease MazF	-
CPF11_RS13835	93320..93520	+	201	WP_012813212.1	hypothetical protein	-
CPF11_RS13840	93550..93870	+	321	WP_012813211.1	hypothetical protein	-
CPF11_RS13845	93927..94331	-	405	WP_006115557.1	hypothetical protein	-
CPF11_RS13850	94344..95159	-	816	WP_006115558.1	hypothetical protein	-
CPF11_RS13855	95256..95720	-	465	WP_039891560.1	hypothetical protein	-
CPF11_RS13860	95723..96571	-	849	WP_039891563.1	hypothetical protein	-
CPF11_RS13865	96688..97476	-	789	WP_006115561.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..131455	131455

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-87)

Antitoxin

• SpoVT-AbrB domain (16-54)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 10974.72 Da
• Isoelectric Point: 4.4353

>T85831 WP_006115553.1 NZ_CP023658:92271-92579 [Acetobacter pomorum]
VSIYLLDTNIISDLVRNPSGSCAQRIEHIDPGDLCTSIIVAAELRYGCAKKGSEKLLNRVESVLAFIPVLPLDIPADCEY
GGIRAELEAAGQTIGMLEKLFA

Nucleotide

• Length: 309 bp

>T85831 NZ_CP023658:92271-92579 [Acetobacter pomorum]
GTGAGCATCTACCTTCTTGATACAAACATCATCAGCGATCTCGTGCGCAATCCTTCCGGCTCCTGTGCGCAACGCATTGA
GCATATTGACCCAGGTGATCTTTGCACCAGCATCATCGTGGCCGCAGAATTACGCTATGGATGCGCCAAAAAAGGCTCCG
AAAAGCTCTTGAACCGGGTCGAAAGCGTGTTGGCGTTTATCCCTGTGCTGCCACTCGATATTCCTGCCGACTGTGAATAC
GGTGGCATCCGCGCCGAACTCGAGGCGGCAGGGCAGACAATCGGCATGCTTGAGAAGCTTTTTGCGTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10440.04 Da
• Isoelectric Point: 4.5749

>AT85831 WP_006115552.1 NZ_CP023658:92002-92274 [Acetobacter pomorum]
MPVPEVTPRPREVKLFRNNRSQAVRIPVEFEMPGDRVLIRRDGDKLVLEPVKTPSTLKELLMAWREEPQLPPEDDFPDIQ
DVAARPEDIL

Nucleotide

• Length: 273 bp

>AT85831 NZ_CP023658:92002-92274 [Acetobacter pomorum]
ATGCCAGTTCCTGAAGTGACCCCCAGACCGCGCGAAGTAAAACTCTTTCGGAATAATCGCAGTCAGGCCGTGCGGATTCC
GGTCGAATTTGAAATGCCGGGAGATCGTGTGCTGATCCGCCGTGACGGTGACAAACTCGTTCTGGAGCCTGTGAAGACGC
CTTCCACGCTGAAGGAGCTTCTCATGGCATGGCGTGAAGAGCCGCAGCTGCCTCCCGAAGACGATTTTCCCGATATTCAG
GATGTTGCCGCCAGACCAGAAGATATCCTGTGA

Structures

Toxin

• AlphaFold DB: A0A401X8Y0

Antitoxin

• AlphaFold DB: A0A401X8Q2