Detailed information of TA system TA085830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 38197..38747
• Replicon: plasmid pApBDGP5A
• Accession: NZ_CP023658
• Organism: Acetobacter pomorum strain BDGP5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: C7JII7
• Locus tag: CPF11_RS13550
• Protein ID: WP_003618711.1
• Coordinates: 38197..38490 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D5QDQ9
• Locus tag: CPF11_RS13555
• Protein ID: WP_003618686.1
• Coordinates: 38487..38747 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPF11_RS13520	33298..33783	+	486	WP_082823248.1	helix-turn-helix domain-containing protein	-
CPF11_RS13525	33908..35518	-	1611	WP_006115450.1	IS66 family transposase	-
CPF11_RS13530	35582..35929	-	348	WP_012812355.1	IS66 family insertion sequence element accessory protein TnpB	-
CPF11_RS13535	35926..36285	-	360	WP_012812356.1	transposase	-
CPF11_RS13540	36384..36803	-	420	Protein_30	DUF1275 domain-containing protein	-
CPF11_RS13545	36803..38065	-	1263	WP_006115449.1	sodium:proton antiporter	-
CPF11_RS13550	38197..38490	-	294	WP_003618711.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
CPF11_RS13555	38487..38747	-	261	WP_003618686.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CPF11_RS13560	39340..40413	-	1074	WP_006115447.1	alkene reductase	-
CPF11_RS13565	40613..41080	-	468	WP_050793094.1	hypothetical protein	-
CPF11_RS13575	42068..42802	-	735	WP_039891505.1	class I SAM-dependent methyltransferase	-
CPF11_RS13580	42870..43310	-	441	WP_039891503.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..131455	131455
	-	flank	IS/Tn	-	-	35582..35929	347

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11605.35 Da
• Isoelectric Point: 7.9998

>T85830 WP_003618711.1 NZ_CP023658:c38490-38197 [Acetobacter pomorum]
MKEYCLEFDDRALREWDALDGSVRKKFEKKLEKLIWNPHSPGNELHRDLSGFYKIKLLKDGYRLVYQVIDERIVIYVIAV
GRRADNEIYELASKRTE

Nucleotide

• Length: 294 bp

>T85830 NZ_CP023658:c38490-38197 [Acetobacter pomorum]
ATGAAAGAGTATTGTCTTGAATTCGACGACAGAGCACTCAGAGAGTGGGATGCTCTTGACGGCAGCGTGCGTAAGAAATT
TGAAAAGAAGCTGGAAAAGCTGATCTGGAACCCTCACTCTCCAGGAAACGAACTGCATCGCGACCTATCAGGGTTTTATA
AGATCAAGCTTCTGAAAGATGGTTACCGCCTTGTTTATCAGGTCATCGACGAACGGATCGTCATCTATGTAATTGCCGTT
GGCCGGAGAGCCGATAATGAGATTTATGAACTGGCTTCGAAGCGAACGGAGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9762.23 Da
• Isoelectric Point: 10.4645

>AT85830 WP_003618686.1 NZ_CP023658:c38747-38487 [Acetobacter pomorum]
MSAVTFRVDETLKAAAVKKLSAQGISLSDALRDTLAYIAETGRSPVKRRLVTDEDAELIEIVRERLKNPAQKHRMTFAEL
KNRHRE

Nucleotide

• Length: 261 bp

>AT85830 NZ_CP023658:c38747-38487 [Acetobacter pomorum]
ATGTCTGCTGTTACATTCCGGGTAGATGAAACGCTGAAGGCAGCCGCAGTTAAGAAGTTGTCGGCTCAAGGCATTTCACT
TTCTGACGCACTGCGCGATACGCTCGCGTATATTGCTGAAACGGGTCGCTCTCCCGTCAAGCGGAGGCTTGTCACAGACG
AGGATGCGGAGCTTATCGAGATTGTGCGCGAACGCCTAAAAAACCCTGCCCAAAAGCATCGTATGACTTTTGCAGAGCTT
AAGAACCGTCATCGCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6N3T9M6