Detailed information of TA system TA085829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2022840..2023423
• Replicon: chromosome
• Accession: NZ_CP023657
• Organism: Acetobacter pomorum strain BDGP5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: F1YSB1
• Locus tag: CPF11_RS09545
• Protein ID: WP_006115878.1
• Coordinates: 2023079..2023423 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: F1YSB0
• Locus tag: CPF11_RS09540
• Protein ID: WP_006115877.1
• Coordinates: 2022840..2023082 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPF11_RS09515	2018077..2018280	-	204	WP_035352404.1	hypothetical protein	-
CPF11_RS09520	2018698..2019321	-	624	WP_035352402.1	hypothetical protein	-
CPF11_RS09525	2019426..2019647	-	222	WP_006115875.1	hypothetical protein	-
CPF11_RS09530	2020326..2020832	+	507	WP_139018862.1	hypothetical protein	-
CPF11_RS09535	2021142..2022533	-	1392	WP_035352412.1	site-specific integrase	-
CPF11_RS09540	2022840..2023082	+	243	WP_006115877.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CPF11_RS09545	2023079..2023423	+	345	WP_006115878.1	endoribonuclease MazF	Toxin
CPF11_RS09550	2023623..2023946	+	324	WP_139018863.1	hypothetical protein	-
CPF11_RS09555	2024254..2025336	-	1083	Protein_1855	site-specific integrase	-
CPF11_RS09560	2025415..2026506	+	1092	WP_035352416.1	IS5 family transposase	-
CPF11_RS14380	2026643..2026813	-	171	Protein_1857	integrase	-
CPF11_RS09565	2026890..2027972	+	1083	WP_170315297.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2025415..2026506	1091
	-	inside	Integrative and Conjugative Element	-	-	2018367..2054301	35934

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (13-112)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12544.68 Da
• Isoelectric Point: 10.4109

>T85829 WP_006115878.1 NZ_CP023657:2023079-2023423 [Acetobacter pomorum]
VSAKGTAVWVPDRGDIVWLEFDPQTGREQAGHRPAIVLSPASYNAKAGMMLCCPTTTKIKGYPFEVALTGKTASVVLSDQ
LRSLDWRVRKAKLKGKVLPEELEAIRQRVRLLVG

Nucleotide

• Length: 345 bp

>T85829 NZ_CP023657:2023079-2023423 [Acetobacter pomorum]
GTGAGTGCCAAAGGAACGGCTGTCTGGGTTCCTGATCGCGGGGACATTGTCTGGCTTGAGTTTGATCCGCAGACGGGCAG
GGAACAGGCCGGGCACCGGCCTGCTATTGTGCTGAGTCCTGCCAGTTATAACGCTAAAGCCGGAATGATGCTGTGCTGCC
CAACAACGACCAAAATCAAAGGCTACCCCTTTGAAGTGGCTCTAACTGGAAAAACTGCCAGTGTGGTTTTGTCAGACCAG
CTTAGAAGTCTGGATTGGCGCGTGAGAAAGGCAAAGCTCAAAGGAAAAGTTTTACCAGAAGAGCTTGAGGCCATCCGGCA
GCGGGTCAGATTGCTGGTCGGGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8736.96 Da
• Isoelectric Point: 4.3094

>AT85829 WP_006115877.1 NZ_CP023657:2022840-2023082 [Acetobacter pomorum]
MQGVVRKWGNSAAIRLPAGVLEAVSLKIDQAVDVREEDGRIIIEPVKTVPLKLEELVSGITAENRYDEMDFGPSVGGESW

Nucleotide

• Length: 243 bp

>AT85829 NZ_CP023657:2022840-2023082 [Acetobacter pomorum]
ATGCAGGGCGTTGTCAGGAAATGGGGAAACAGTGCAGCCATTCGTCTTCCTGCTGGTGTGCTTGAAGCCGTCAGTCTGAA
GATCGATCAGGCTGTTGATGTGCGGGAAGAAGATGGGCGCATTATCATTGAGCCAGTCAAAACAGTTCCTCTCAAACTGG
AAGAATTAGTGTCAGGCATTACGGCCGAGAACCGCTATGATGAAATGGACTTTGGTCCGTCTGTGGGTGGGGAAAGCTGG
TGA

Structures

Toxin

• AlphaFold DB: F1YSB1

Antitoxin

• AlphaFold DB: F1YSB0