Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-YafN |
| Location | 16796..17331 | Replicon | plasmid pCFSAN030807_1 |
| Accession | NZ_CP023646 | ||
| Organism | Shigella sonnei strain CFSAN030807 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | Q3YTD6 |
| Locus tag | AT852_RS26565 | Protein ID | WP_000222766.1 |
| Coordinates | 17044..17331 (+) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | A0A0I0JNN8 |
| Locus tag | AT852_RS26560 | Protein ID | WP_001132903.1 |
| Coordinates | 16796..17047 (+) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AT852_RS26500 (AT852_26500) | 12062..12523 | + | 462 | WP_001233865.1 | thermonuclease family protein | - |
| AT852_RS26505 (AT852_26505) | 12569..12778 | + | 210 | WP_001299729.1 | hemolysin expression modulator Hha | - |
| AT852_RS26510 (AT852_26510) | 12817..13062 | + | 246 | Protein_13 | hypothetical protein | - |
| AT852_RS26525 (AT852_26525) | 13831..14184 | + | 354 | Protein_15 | DUF2726 domain-containing protein | - |
| AT852_RS26530 (AT852_26530) | 14424..14684 | + | 261 | WP_000083819.1 | replication regulatory protein RepA | - |
| AT852_RS26540 (AT852_26540) | 14908..14982 | + | 75 | WP_001365705.1 | RepA leader peptide Tap | - |
| AT852_RS26545 (AT852_26545) | 14975..15832 | + | 858 | WP_000130974.1 | incFII family plasmid replication initiator RepA | - |
| AT852_RS29025 (AT852_26555) | 16532..16723 | + | 192 | WP_000829591.1 | hypothetical protein | - |
| AT852_RS26560 (AT852_26560) | 16796..17047 | + | 252 | WP_001132903.1 | plasmid stabilization protein | Antitoxin |
| AT852_RS26565 (AT852_26565) | 17044..17331 | + | 288 | WP_000222766.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| AT852_RS26570 (AT852_26570) | 17564..18763 | - | 1200 | WP_000947184.1 | IS91 family transposase | - |
| AT852_RS29030 (AT852_26575) | 18773..18961 | - | 189 | WP_000957853.1 | hypothetical protein | - |
| AT852_RS26580 (AT852_26580) | 19083..19241 | + | 159 | Protein_24 | transposase | - |
| AT852_RS26585 (AT852_26585) | 19380..19535 | + | 156 | Protein_25 | IS256 family transposase | - |
| AT852_RS26590 (AT852_26590) | 19565..21292 | - | 1728 | WP_000597732.1 | T3SS effector E3 ubiquitin-protein ligase IpaH1.4 | - |
| AT852_RS26595 (AT852_26595) | 21682..21901 | - | 220 | Protein_27 | hypothetical protein | - |
| AT852_RS26600 (AT852_26600) | 22058..22155 | - | 98 | Protein_28 | IS1 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | ipaH1.4 / tviC / nleE / icsP/sopA / ospB / ospC4 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ospC1 / ospD3/senA / ipaH7.8 / ipaH4.5 / ospC2 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG | 1..214673 | 214673 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11147.23 Da Isoelectric Point: 10.5832
>T85819 WP_000222766.1 NZ_CP023646:17044-17331 [Shigella sonnei]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR
Download Length: 288 bp
>T85819 NZ_CP023646:17044-17331 [Shigella sonnei]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCGGCAAAACTAAGAGGGCTAAAGGACTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCGGCAAAACTAAGAGGGCTAAAGGACTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG
Antitoxin
Download Length: 84 a.a. Molecular weight: 9225.44 Da Isoelectric Point: 4.0927
>AT85819 WP_001132903.1 NZ_CP023646:16796-17047 [Shigella sonnei]
MPNIILSETSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL
MPNIILSETSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL
Download Length: 252 bp
>AT85819 NZ_CP023646:16796-17047 [Shigella sonnei]
ATGCCTAACATCATTCTGTCTGAGACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA
ATGCCTAACATCATTCTGTCTGAGACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CDZ2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0I0JNN8 |