Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 16796..17331 Replicon plasmid pCFSAN030807_1
Accession NZ_CP023646
Organism Shigella sonnei strain CFSAN030807

Toxin (Protein)


Gene name relE Uniprot ID Q3YTD6
Locus tag AT852_RS26565 Protein ID WP_000222766.1
Coordinates 17044..17331 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0I0JNN8
Locus tag AT852_RS26560 Protein ID WP_001132903.1
Coordinates 16796..17047 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AT852_RS26500 (AT852_26500) 12062..12523 + 462 WP_001233865.1 thermonuclease family protein -
AT852_RS26505 (AT852_26505) 12569..12778 + 210 WP_001299729.1 hemolysin expression modulator Hha -
AT852_RS26510 (AT852_26510) 12817..13062 + 246 Protein_13 hypothetical protein -
AT852_RS26525 (AT852_26525) 13831..14184 + 354 Protein_15 DUF2726 domain-containing protein -
AT852_RS26530 (AT852_26530) 14424..14684 + 261 WP_000083819.1 replication regulatory protein RepA -
AT852_RS26540 (AT852_26540) 14908..14982 + 75 WP_001365705.1 RepA leader peptide Tap -
AT852_RS26545 (AT852_26545) 14975..15832 + 858 WP_000130974.1 incFII family plasmid replication initiator RepA -
AT852_RS29025 (AT852_26555) 16532..16723 + 192 WP_000829591.1 hypothetical protein -
AT852_RS26560 (AT852_26560) 16796..17047 + 252 WP_001132903.1 plasmid stabilization protein Antitoxin
AT852_RS26565 (AT852_26565) 17044..17331 + 288 WP_000222766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AT852_RS26570 (AT852_26570) 17564..18763 - 1200 WP_000947184.1 IS91 family transposase -
AT852_RS29030 (AT852_26575) 18773..18961 - 189 WP_000957853.1 hypothetical protein -
AT852_RS26580 (AT852_26580) 19083..19241 + 159 Protein_24 transposase -
AT852_RS26585 (AT852_26585) 19380..19535 + 156 Protein_25 IS256 family transposase -
AT852_RS26590 (AT852_26590) 19565..21292 - 1728 WP_000597732.1 T3SS effector E3 ubiquitin-protein ligase IpaH1.4 -
AT852_RS26595 (AT852_26595) 21682..21901 - 220 Protein_27 hypothetical protein -
AT852_RS26600 (AT852_26600) 22058..22155 - 98 Protein_28 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ipaH1.4 / tviC / nleE / icsP/sopA / ospB / ospC4 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ospC1 / ospD3/senA / ipaH7.8 / ipaH4.5 / ospC2 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG 1..214673 214673


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11147.23 Da        Isoelectric Point: 10.5832

>T85819 WP_000222766.1 NZ_CP023646:17044-17331 [Shigella sonnei]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T85819 NZ_CP023646:17044-17331 [Shigella sonnei]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCGGCAAAACTAAGAGGGCTAAAGGACTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9225.44 Da        Isoelectric Point: 4.0927

>AT85819 WP_001132903.1 NZ_CP023646:16796-17047 [Shigella sonnei]
MPNIILSETSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT85819 NZ_CP023646:16796-17047 [Shigella sonnei]
ATGCCTAACATCATTCTGTCTGAGACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDZ2

Antitoxin

Source ID Structure
AlphaFold DB A0A0I0JNN8

References