Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4078826..4079323 Replicon chromosome
Accession NZ_CP023645
Organism Shigella sonnei strain CFSAN030807

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AT852_RS22590 Protein ID WP_001173343.1
Coordinates 4078826..4078999 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag AT852_RS22595 Protein ID WP_000398619.1
Coordinates 4079027..4079323 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AT852_RS22570 (4073978) 4073978..4075134 + 1157 WP_087879933.1 IS3-like element IS600 family transposase -
AT852_RS22575 (4075180) 4075180..4075437 + 258 Protein_4063 lysine decarboxylase LdcC -
AT852_RS22580 (4075496) 4075496..4076953 + 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
AT852_RS22585 (4077190) 4077190..4078707 + 1518 WP_001295086.1 lysine--tRNA ligase -
AT852_RS22590 (4078826) 4078826..4078999 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AT852_RS22595 (4079027) 4079027..4079323 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AT852_RS22605 (4079550) 4079550..4079822 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
AT852_RS22610 (4079834) 4079834..4080064 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
AT852_RS22615 (4080245) 4080245..4081876 + 1632 WP_001216495.1 sensor histidine kinase -
AT852_RS22620 (4081873) 4081873..4082592 + 720 WP_000611285.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T85817 WP_001173343.1 NZ_CP023645:c4078999-4078826 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T85817 NZ_CP023645:c4078999-4078826 [Shigella sonnei]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT85817 WP_000398619.1 NZ_CP023645:c4079323-4079027 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT85817 NZ_CP023645:c4079323-4079027 [Shigella sonnei]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References