Detailed information of TA system TA085815

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3389590..3390427
• Replicon: chromosome
• Accession: NZ_CP023645
• Organism: Shigella sonnei strain CFSAN030807

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: AT852_RS18950
• Protein ID: WP_000227784.1
• Coordinates: 3389885..3390427 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: AT852_RS18945
• Protein ID: WP_001297137.1
• Coordinates: 3389590..3389901 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AT852_RS18920 (3384610)	3384610..3385557	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
AT852_RS18925 (3385579)	3385579..3387570	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AT852_RS18930 (3387560)	3387560..3388174	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AT852_RS18935 (3388174)	3388174..3388503	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AT852_RS18940 (3388515)	3388515..3389405	+	891	WP_000971327.1	heme o synthase	-
AT852_RS18945 (3389590)	3389590..3389901	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
AT852_RS18950 (3389885)	3389885..3390427	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
AT852_RS18955 (3390483)	3390483..3391049	-	567	Protein_3404	SEL1-like repeat protein	-
AT852_RS18965 (3391083)	3391083..3391780	+	698	WP_237158184.1	IS1-like element IS1A family transposase	-
AT852_RS18970 (3391797)	3391797..3392195	-	399	Protein_3406	SEL1-like repeat protein	-
AT852_RS18985 (3393380)	3393380..3394744	+	1365	WP_001000971.1	MFS transporter	-
AT852_RS18990 (3394872)	3394872..3395363	-	492	WP_001138904.1	nucleotide binding protein YajQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T85815 WP_000227784.1 NZ_CP023645:3389885-3390427 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T85815 NZ_CP023645:3389885-3390427 [Shigella sonnei]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT85815 WP_001297137.1 NZ_CP023645:3389590-3389901 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT85815 NZ_CP023645:3389590-3389901 [Shigella sonnei]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3