Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3352114..3352732 Replicon chromosome
Accession NZ_CP023645
Organism Shigella sonnei strain CFSAN030807

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AT852_RS18760 Protein ID WP_001291435.1
Coordinates 3352514..3352732 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0H8V4S8
Locus tag AT852_RS18755 Protein ID WP_000344804.1
Coordinates 3352114..3352488 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AT852_RS18745 (3347203) 3347203..3348396 + 1194 WP_011310152.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AT852_RS18750 (3348419) 3348419..3351568 + 3150 WP_001132464.1 efflux RND transporter permease AcrB -
AT852_RS18755 (3352114) 3352114..3352488 + 375 WP_000344804.1 Hha toxicity modulator TomB Antitoxin
AT852_RS18760 (3352514) 3352514..3352732 + 219 WP_001291435.1 HHA domain-containing protein Toxin
AT852_RS18765 (3352903) 3352903..3353454 + 552 WP_000102564.1 maltose O-acetyltransferase -
AT852_RS18770 (3353570) 3353570..3354040 + 471 WP_096098080.1 YlaC family protein -
AT852_RS18775 (3354204) 3354204..3355752 + 1549 Protein_3368 cyclic-guanylate-specific phosphodiesterase PdeB -
AT852_RS18780 (3355794) 3355794..3356147 - 354 WP_000878140.1 DUF1428 domain-containing protein -
AT852_RS18790 (3356526) 3356526..3356837 + 312 WP_000409911.1 MGMT family protein -
AT852_RS18795 (3356868) 3356868..3357440 - 573 WP_000779812.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T85814 WP_001291435.1 NZ_CP023645:3352514-3352732 [Shigella sonnei]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T85814 NZ_CP023645:3352514-3352732 [Shigella sonnei]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14615.43 Da        Isoelectric Point: 4.6232

>AT85814 WP_000344804.1 NZ_CP023645:3352114-3352488 [Shigella sonnei]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT85814 NZ_CP023645:3352114-3352488 [Shigella sonnei]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGAGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H8V4S8

References