Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3070517..3071204 Replicon chromosome
Accession NZ_CP023640
Organism Mycobacterium tuberculosis strain TBV4952

Toxin (Protein)


Gene name vapC Uniprot ID G0TF65
Locus tag CP497_RS15095 Protein ID WP_003414064.1
Coordinates 3070809..3071204 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag CP497_RS15090 Protein ID WP_003414061.1
Coordinates 3070517..3070783 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP497_RS15065 3066156..3067058 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
CP497_RS15070 3067127..3067879 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
CP497_RS15075 3068123..3068398 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
CP497_RS15080 3068395..3070017 - 1623 WP_003414057.1 type I restriction-modification system subunit M -
CP497_RS15085 3070104..3070520 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 -
CP497_RS15090 3070517..3070783 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CP497_RS15095 3070809..3071204 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin Toxin
CP497_RS15100 3071201..3071470 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
CP497_RS15105 3071480..3072574 - 1095 WP_003900565.1 restriction endonuclease subunit S -
CP497_RS15110 3072571..3072990 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
CP497_RS15115 3073064..3073543 - 480 WP_003414073.1 dihydrofolate reductase -
CP497_RS15120 3073614..3074414 - 801 WP_003911953.1 thymidylate synthase -
CP497_RS15125 3074570..3075307 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-125)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 132 a.a.        Molecular weight: 14340.04 Da        Isoelectric Point: 4.7145

>T85786 WP_003414064.1 NZ_CP023640:c3071204-3070809 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Download         Length: 396 bp

>T85786 NZ_CP023640:c3071204-3070809 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT85786 WP_003414061.1 NZ_CP023640:c3070783-3070517 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT85786 NZ_CP023640:c3070783-3070517 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G0TF65


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References