Detailed information of TA system TA085785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3070104..3070783
• Replicon: chromosome
• Accession: NZ_CP023640
• Organism: Mycobacterium tuberculosis strain TBV4952

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: CP497_RS15085
• Protein ID: WP_003414059.1
• Coordinates: 3070104..3070520 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: CP497_RS15090
• Protein ID: WP_003414061.1
• Coordinates: 3070517..3070783 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP497_RS15065	3066156..3067058	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CP497_RS15070	3067127..3067879	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
CP497_RS15075	3068123..3068398	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
CP497_RS15080	3068395..3070017	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
CP497_RS15085	3070104..3070520	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
CP497_RS15090	3070517..3070783	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CP497_RS15095	3070809..3071204	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
CP497_RS15100	3071201..3071470	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
CP497_RS15105	3071480..3072574	-	1095	WP_003900565.1	restriction endonuclease subunit S	-
CP497_RS15110	3072571..3072990	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
CP497_RS15115	3073064..3073543	-	480	WP_003414073.1	dihydrofolate reductase	-
CP497_RS15120	3073614..3074414	-	801	WP_003911953.1	thymidylate synthase	-
CP497_RS15125	3074570..3075307	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T85785 WP_003414059.1 NZ_CP023640:c3070520-3070104 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T85785 NZ_CP023640:c3070520-3070104 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT85785 WP_003414061.1 NZ_CP023640:c3070783-3070517 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT85785 NZ_CP023640:c3070783-3070517 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64