Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2194591..2195294 Replicon chromosome
Accession NZ_CP023640
Organism Mycobacterium tuberculosis strain TBV4952

Toxin (Protein)


Gene name mazF Uniprot ID G0TLK7
Locus tag CP497_RS10545 Protein ID WP_003409778.1
Coordinates 2194591..2194920 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TLK8
Locus tag CP497_RS10550 Protein ID WP_003409780.1
Coordinates 2194917..2195294 (-) Length 126 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP497_RS10525 2190974..2192044 + 1071 WP_003899091.1 epoxide hydrolase EphB -
CP497_RS10530 2192041..2192556 + 516 WP_003409718.1 flavin reductase family protein -
CP497_RS10535 2192553..2193614 + 1062 WP_003899092.1 3,4-dihydroxy-2-butanone-4-phosphate synthase -
CP497_RS10540 2193611..2194381 + 771 WP_003409775.1 SDR family oxidoreductase -
CP497_RS10545 2194591..2194920 - 330 WP_003409778.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CP497_RS10550 2194917..2195294 - 378 WP_003409780.1 type II toxin-antitoxin system antitoxin MazE5 Antitoxin
CP497_RS10555 2195291..2195881 - 591 WP_003409784.1 SEC-C domain-containing protein -
CP497_RS10560 2195936..2197300 + 1365 WP_079028028.1 HNH endonuclease -
CP497_RS10565 2197455..2197907 - 453 WP_003899095.1 lipoprotein -
CP497_RS10570 2197971..2198372 + 402 WP_003409869.1 hypothetical protein -
CP497_RS10575 2198365..2198547 - 183 WP_003409870.1 hypothetical protein -
CP497_RS10580 2198661..2199011 - 351 WP_003899096.1 hypothetical protein -
CP497_RS10585 2199022..2199924 - 903 WP_003899097.1 hypothetical protein -
CP497_RS10590 2199945..2200136 - 192 WP_003409876.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-103)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 110 a.a.        Molecular weight: 11787.82 Da        Isoelectric Point: 8.5361

>T85767 WP_003409778.1 NZ_CP023640:c2194920-2194591 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Download         Length: 330 bp

>T85767 NZ_CP023640:c2194920-2194591 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13560.07 Da        Isoelectric Point: 5.1519

>AT85767 WP_003409780.1 NZ_CP023640:c2195294-2194917 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Download         Length: 378 bp

>AT85767 NZ_CP023640:c2195294-2194917 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G0TLK7


Antitoxin

Source ID Structure
AlphaFold DB G0TLK8

References