Detailed information of TA system TA085649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2225252..2225940
• Replicon: chromosome
• Accession: NZ_CP023638
• Organism: Mycobacterium tuberculosis strain TBV4766

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CP495_RS10770
• Protein ID: WP_096877036.1
• Coordinates: 2225252..2225671 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ28
• Locus tag: CP495_RS10775
• Protein ID: WP_003409968.1
• Coordinates: 2225680..2225940 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP495_RS10750	2220747..2221595	+	849	WP_003409942.1	class I SAM-dependent methyltransferase	-
CP495_RS10755	2221558..2223003	-	1446	WP_003899115.1	APC family permease	-
CP495_RS10760	2223182..2223868	-	687	WP_003409954.1	immunoprotective protein Mpt64	-
CP495_RS10765	2224059..2225027	-	969	WP_003899116.1	class 1b ribonucleoside-diphosphate reductase subunit beta	-
CP495_RS10770	2225252..2225671	-	420	WP_096877036.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP495_RS10775	2225680..2225940	-	261	WP_003409968.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CP495_RS10780	2226083..2227759	+	1677	WP_003899118.1	PecA family PE domain-processing aspartic protease	-
CP495_RS10785	2227747..2228400	-	654	WP_003409976.1	cutinase Cfp21	-
CP495_RS10790	2228515..2228745	+	231	WP_003409980.1	DUF167 domain-containing protein	-
CP495_RS10795	2228830..2229741	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
CP495_RS10800	2229850..2230449	+	600	WP_003409989.1	L-lysine exporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Antitoxin VapB-like (2-81)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14690.88 Da
• Isoelectric Point: 7.9535

>T85649 WP_096877036.1 NZ_CP023638:c2225671-2225252 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLELRGVVGYWPGVA

Nucleotide

• Length: 420 bp

>T85649 NZ_CP023638:c2225671-2225252 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTACGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9528.88 Da
• Isoelectric Point: 5.1007

>AT85649 WP_003409968.1 NZ_CP023638:c2225940-2225680 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Nucleotide

• Length: 261 bp

>AT85649 NZ_CP023638:c2225940-2225680 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829C483