Detailed information of TA system TA085489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3707477..3708151
• Replicon: chromosome
• Accession: NZ_CP023635
• Organism: Mycobacterium tuberculosis strain TBDM2489

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: CP492_RS18190
• Protein ID: WP_003417282.1
• Coordinates: 3707477..3707905 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: CP492_RS18195
• Protein ID: WP_003417286.1
• Coordinates: 3707909..3708151 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP492_RS18165	3703299..3703700	-	402	WP_003417264.1	cytidine deaminase	-
CP492_RS18170	3703841..3704275	+	435	WP_003900017.1	succinate dehydrogenase, cytochrome b556 subunit	-
CP492_RS18175	3704272..3704706	+	435	WP_003417269.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
CP492_RS18180	3704835..3706607	+	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
CP492_RS18185	3706607..3707398	+	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
CP492_RS18190	3707477..3707905	-	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP492_RS18195	3707909..3708151	-	243	WP_003417286.1	ribbon-helix-helix domain-containing protein	Antitoxin
CP492_RS18200	3708273..3708887	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
CP492_RS18205	3708884..3709549	-	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
CP492_RS18210	3709550..3710083	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
CP492_RS21935	3710080..3710232	-	153	WP_003916129.1	hypothetical protein	-
CP492_RS18220	3710268..3711529	+	1262	WP_087902221.1	IS3-like element IS987 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T85489 WP_003417282.1 NZ_CP023635:c3707905-3707477 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T85489 NZ_CP023635:c3707905-3707477 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT85489 WP_003417286.1 NZ_CP023635:c3708151-3707909 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT85489 NZ_CP023635:c3708151-3707909 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59