Detailed information of TA system TA085456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 1388593..1389152
• Replicon: chromosome
• Accession: NZ_CP023635
• Organism: Mycobacterium tuberculosis strain TBDM2489

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0THS1
• Locus tag: CP492_RS06825
• Protein ID: WP_003898789.1
• Coordinates: 1388593..1388886 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0THS2
• Locus tag: CP492_RS06830
• Protein ID: WP_003406322.1
• Coordinates: 1388883..1389152 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP492_RS06795	1384186..1384446	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	-
CP492_RS06800	1384443..1384874	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	-
CP492_RS06805	1384897..1386585	-	1689	WP_003910308.1	PE family protein	-
CP492_RS06815	1386765..1387625	+	861	WP_003900301.1	hypothetical protein	-
CP492_RS06820	1387706..1388536	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
CP492_RS06825	1388593..1388886	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
CP492_RS06830	1388883..1389152	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CP492_RS06835	1389265..1392960	-	3696	WP_009938349.1	multifunctional oxoglutarate decarboxylase/oxoglutarate dehydrogenase thiamine pyrophosphate-binding subunit/dihydrolipoyllysine-residue succinyltransferase subunit	-
CP492_RS06840	1393102..1393890	-	789	WP_003406325.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (9-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11031.57 Da
• Isoelectric Point: 9.6275

>T85456 WP_003898789.1 NZ_CP023635:c1388886-1388593 [Mycobacterium tuberculosis]
VSDDHPYHVAITATAARDLQRLPEKIAAACVEFVFGPLLNNPHRLGKPLRNDLEGLHSARRGDYRVVYAIDDGHHRVEII
HIARRSASYRMNPCRPR

Nucleotide

• Length: 294 bp

>T85456 NZ_CP023635:c1388886-1388593 [Mycobacterium tuberculosis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9766.97 Da
• Isoelectric Point: 4.8615

>AT85456 WP_003406322.1 NZ_CP023635:c1389152-1388883 [Mycobacterium tuberculosis]
MAVVPLGEVRNRLSEYVAEVELTHERITITRHGHPAAVLISADDLASIEETLEVLRTPGASEAIREGLADVAAGRFVSND
EIRNRYTAR

Nucleotide

• Length: 270 bp

>AT85456 NZ_CP023635:c1389152-1388883 [Mycobacterium tuberculosis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Structures

Toxin

• AlphaFold DB: G0THS1

Antitoxin

• AlphaFold DB: G0THS2