Detailed information of TA system TA085428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3707483..3708157
• Replicon: chromosome
• Accession: NZ_CP023634
• Organism: Mycobacterium tuberculosis strain TBDM2487

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: CP491_RS18185
• Protein ID: WP_003417282.1
• Coordinates: 3707483..3707911 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: CP491_RS18190
• Protein ID: WP_003417286.1
• Coordinates: 3707915..3708157 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP491_RS18160	3703305..3703706	-	402	WP_003417264.1	cytidine deaminase	-
CP491_RS18165	3703847..3704281	+	435	WP_003900017.1	succinate dehydrogenase, cytochrome b556 subunit	-
CP491_RS18170	3704278..3704712	+	435	WP_003417269.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
CP491_RS18175	3704841..3706613	+	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
CP491_RS18180	3706613..3707404	+	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
CP491_RS18185	3707483..3707911	-	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP491_RS18190	3707915..3708157	-	243	WP_003417286.1	ribbon-helix-helix domain-containing protein	Antitoxin
CP491_RS18195	3708279..3708893	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
CP491_RS18200	3708890..3709555	-	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
CP491_RS18205	3709556..3710089	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
CP491_RS21950	3710086..3710238	-	153	WP_003916129.1	hypothetical protein	-
CP491_RS18215	3710274..3711535	+	1262	WP_087902221.1	IS3-like element IS987 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T85428 WP_003417282.1 NZ_CP023634:c3707911-3707483 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T85428 NZ_CP023634:c3707911-3707483 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT85428 WP_003417286.1 NZ_CP023634:c3708157-3707915 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT85428 NZ_CP023634:c3708157-3707915 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59