Detailed information of TA system TA085426

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3183003..3183690
• Replicon: chromosome
• Accession: NZ_CP023634
• Organism: Mycobacterium tuberculosis strain TBDM2487

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CP491_RS15695
• Protein ID: WP_086151622.1
• Coordinates: 3183247..3183690 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: CP491_RS15690
• Protein ID: WP_003414620.1
• Coordinates: 3183003..3183260 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP491_RS15670	3178323..3179177	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
CP491_RS15675	3179233..3180396	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
CP491_RS15680	3180413..3181627	-	1215	WP_003899518.1	zinc metalloprotease Rip	-
CP491_RS15685	3181635..3182876	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
CP491_RS15690	3183003..3183260	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
CP491_RS15695	3183247..3183690	+	444	WP_086151622.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP491_RS15700	3183770..3184432	+	663	WP_086151623.1	cell surface glycolipoprotein Mpt83	-
CP491_RS15705	3184528..3184719	+	192	WP_003414632.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
CP491_RS15710	3185051..3186799	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
CP491_RS15715	3186895..3187476	+	582	WP_003414644.1	fasciclin domain-containing protein	-
CP491_RS15720	3187576..3187842	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16581.69 Da
• Isoelectric Point: 6.4886

>T85426 WP_086151622.1 NZ_CP023634:3183247-3183690 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDDPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T85426 NZ_CP023634:3183247-3183690 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGACCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT85426 WP_003414620.1 NZ_CP023634:3183003-3183260 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT85426 NZ_CP023634:3183003-3183260 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Antitoxin

• AlphaFold DB: G0TFH0