Detailed information of TA system TA085418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2929935..2930649
• Replicon: chromosome
• Accession: NZ_CP023634
• Organism: Mycobacterium tuberculosis strain TBDM2487

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQK0
• Locus tag: CP491_RS14255
• Protein ID: WP_003413460.1
• Coordinates: 2930209..2930649 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CP491_RS14250
• Protein ID: WP_030030794.1
• Coordinates: 2929935..2930222 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP491_RS14215	2925357..2925602	+	246	WP_003413429.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
CP491_RS14220	2925599..2926003	+	405	WP_003899389.1	type II toxin-antitoxin system VapC family toxin	-
CP491_RS14225	2926220..2926840	+	621	WP_003413441.1	DUF4178 domain-containing protein	-
CP491_RS14230	2926851..2927345	+	495	WP_003413444.1	DUF2617 family protein	-
CP491_RS14235	2927342..2927773	+	432	WP_003899390.1	DUF4247 domain-containing protein	-
CP491_RS14240	2927798..2928256	+	459	WP_003413451.1	DUF350 domain-containing protein	-
CP491_RS14245	2928253..2929824	+	1572	WP_003899392.1	polyamine aminopropyltransferase	-
CP491_RS14250	2929935..2930222	+	288	WP_030030794.1	antitoxin	Antitoxin
CP491_RS14255	2930209..2930649	+	441	WP_003413460.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP491_RS14260	2930670..2931425	-	756	WP_003413464.1	YebC/PmpR family DNA-binding transcriptional regulator	-
CP491_RS14265	2931558..2932154	-	597	WP_003413465.1	pyridoxal 5'-phosphate synthase glutaminase subunit PdxT	-
CP491_RS14270	2932162..2933007	-	846	WP_003413466.1	acyl-CoA thioesterase II	-
CP491_RS14275	2933036..2933935	-	900	WP_003413468.1	pyridoxal 5'-phosphate synthase lyase subunit PdxS	-
CP491_RS14280	2934063..2934737	+	675	WP_003413471.1	pyridoxamine 5'-phosphate oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-146)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 16026.38 Da
• Isoelectric Point: 6.8599

>T85418 WP_003413460.1 NZ_CP023634:2930209-2930649 [Mycobacterium tuberculosis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Nucleotide

• Length: 441 bp

>T85418 NZ_CP023634:2930209-2930649 [Mycobacterium tuberculosis]
ATGCTGCTCTGTGACACCAACATCTGGCTGGCGTTGGCGCTTTCCGGACACGTGCACCACAGGGCCTCGCGCGCATGGCT
AGACACCATCAACGCGCCCGGAGTCATCCACTTTTGCCGCGCAACCCAACAGTCGCTCCTTCGGCTGTTGACGAATCGGA
CGGTGCTGGGCGCGTATGGCAGCCCACCACTGACCAACCGCGAAGCGTGGGCGGCCTATGCCGCGTTCCTGGATGACGAC
CGCATCGTGCTGGCCGGCGCCGAACCTGATGGTTTGGAGGCCCAGTGGAGAGCCTTCGCCGTTCGCCAGTCGCCGGCGCC
CAAGGTTTGGATGGATGCCTACCTAGCTGCTTTCGCACTTACCGGTGGATTCGAGTTGGTGACGACTGACACCGCCTTCA
CCCAGTACGGCGGAATCGAGCTGCGGCTCCTGGCCAAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10512.02 Da
• Isoelectric Point: 6.7173

>AT85418 WP_030030794.1 NZ_CP023634:2929935-2930222 [Mycobacterium tuberculosis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGDAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Nucleotide

• Length: 288 bp

>AT85418 NZ_CP023634:2929935-2930222 [Mycobacterium tuberculosis]
ATGAAGACCACGCTCGACCTGCCTGATGAACTGATGCGCGCTATCAAGGTCCGCGCGGCGCAGCAGGGCCGCAAGATGAA
AGATGTCGTGACCGAACTGCTCAGATCCGGTCTGTCCCAGACGCACAGCGGGGCTCCAATCCCAACGCCGCGGCGCGTGC
AGCTTCCCCTGGTGCATTGCGGTGACGCGGCTACCCGCGAACAAGAAATGACGCCGGAGCGTGTTGCCGCGGCCTTGCTC
GACCAGGAGGCCCAGTGGTGGTCCGGACACGACGATGCTGCTCTGTGA

Structures

Toxin

• AlphaFold DB: G0TQK0