Detailed information of TA system TA085417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2869593..2870230
• Replicon: chromosome
• Accession: NZ_CP023634
• Organism: Mycobacterium tuberculosis strain TBDM2487

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CP491_RS13970
• Protein ID: WP_096876977.1
• Coordinates: 2869593..2869988 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ44
• Locus tag: CP491_RS13975
• Protein ID: WP_003413183.1
• Coordinates: 2869985..2870230 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP491_RS13925	2864996..2866207	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
CP491_RS13935	2866334..2866993	+	660	WP_003900846.1	LppA family lipoprotein	-
CP491_RS13940	2866990..2867652	+	663	WP_003900848.1	hypothetical protein	-
CP491_RS13945	2867649..2867927	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
CP491_RS13950	2868020..2868433	+	414	WP_003413164.1	PIN domain nuclease	-
CP491_RS13955	2868472..2868729	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	-
CP491_RS13960	2868726..2869103	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
CP491_RS13965	2869119..2869493	-	375	WP_003413177.1	hypothetical protein	-
CP491_RS13970	2869593..2869988	-	396	WP_096876977.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	Toxin
CP491_RS13975	2869985..2870230	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	Antitoxin
CP491_RS13985	2870641..2871060	-	420	WP_003413190.1	A24 family peptidase	-
CP491_RS13990	2871072..2871881	-	810	WP_003413193.1	shikimate dehydrogenase	-
CP491_RS13995	2871878..2873131	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
CP491_RS14000	2873124..2873636	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endonuclease VapC20-like (3-127)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-49)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14591.47 Da
• Isoelectric Point: 6.9794

>T85417 WP_096876977.1 NZ_CP023634:c2869988-2869593 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEARPE

Nucleotide

• Length: 396 bp

>T85417 NZ_CP023634:c2869988-2869593 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGCAAGACCCGAGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9098.33 Da
• Isoelectric Point: 5.6007

>AT85417 WP_003413183.1 NZ_CP023634:c2870230-2869985 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Nucleotide

• Length: 246 bp

>AT85417 NZ_CP023634:c2870230-2869985 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BW31