Detailed information of TA system TA085389

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1014554..1015173
• Replicon: chromosome
• Accession: NZ_CP023634
• Organism: Mycobacterium tuberculosis strain TBDM2487

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: CP491_RS04955
• Protein ID: WP_003404726.1
• Coordinates: 1014739..1015173 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: CP491_RS04950
• Protein ID: WP_003898641.1
• Coordinates: 1014554..1014733 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP491_RS04940	1010027..1011607	+	1581	WP_003909313.1	serine hydrolase	-
CP491_RS04945	1011604..1013997	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
CP491_RS21720	1014270..1014428	+	159	WP_003404720.1	hypothetical protein	-
CP491_RS04950	1014554..1014733	+	180	WP_003898641.1	antitoxin	Antitoxin
CP491_RS04955	1014739..1015173	+	435	WP_003404726.1	SRPBCC family protein	Toxin
CP491_RS04960	1015271..1016044	+	774	WP_003404735.1	VOC family protein	-
CP491_RS04965	1016109..1016558	+	450	WP_003404738.1	hypothetical protein	-
CP491_RS04970	1016693..1016923	-	231	WP_003898642.1	hypothetical protein	-
CP491_RS04975	1017090..1018598	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
CP491_RS04980	1018600..1019838	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T85389 WP_003404726.1 NZ_CP023634:1014739-1015173 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T85389 NZ_CP023634:1014739-1015173 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT85389 WP_003898641.1 NZ_CP023634:1014554-1014733 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT85389 NZ_CP023634:1014554-1014733 [Mycobacterium tuberculosis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5