85376

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-CcdA
Location 640119..640795 Replicon chromosome
Accession NZ_CP023634
Organism Mycobacterium tuberculosis strain TBDM2487

Toxin (Protein)


Gene name vapC Uniprot ID G0TPQ9
Locus tag CP491_RS02940 Protein ID WP_003898500.1
Coordinates 640119..640532 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TPR0
Locus tag CP491_RS02945 Protein ID WP_003402915.1
Coordinates 640529..640795 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP491_RS02910 635464..635766 - 303 WP_003402896.1 DUF3349 domain-containing protein -
CP491_RS02915 635826..636104 - 279 WP_003402901.1 hypothetical protein -
CP491_RS02920 636101..637354 - 1254 WP_003911224.1 inorganic phosphate transporter family protein -
CP491_RS02925 637474..637860 - 387 WP_003402907.1 VOC family protein -
CP491_RS02930 637923..638807 - 885 WP_003402909.1 SDR family oxidoreductase -
CP491_RS02935 638903..639805 - 903 WP_003915439.1 1,4-dihydroxy-2-naphthoyl-CoA synthase -
CP491_RS02940 640119..640532 - 414 WP_003898500.1 PIN domain-containing protein Toxin
CP491_RS02945 640529..640795 - 267 WP_003402915.1 antitoxin VapB3 Antitoxin
CP491_RS02950 640987..642702 - 1716 WP_003402917.1 fatty-acid--CoA ligase FadD8 -
CP491_RS02955 642780..644384 + 1605 WP_003402920.1 amidohydrolase -
CP491_RS02960 644381..645361 + 981 WP_003402923.1 o-succinylbenzoate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Pae0151-like, PIN domain

(13-129)

Antitoxin

Post-segregation antitoxin CcdA

(19-61)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 14667.78 Da        Isoelectric Point: 7.3603

>T85376 WP_003898500.1 NZ_CP023634:c640532-640119 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Download         Length: 414 bp

>T85376 NZ_CP023634:c640532-640119 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9543.79 Da        Isoelectric Point: 4.5994

>AT85376 WP_003402915.1 NZ_CP023634:c640795-640529 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Download         Length: 267 bp

>AT85376 NZ_CP023634:c640795-640529 [Mycobacterium tuberculosis]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TPQ9

Antitoxin

Source ID Structure
AlphaFold DB G0TPR0

References