Detailed information of TA system TA085316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 677636..678255
• Replicon: chromosome
• Accession: NZ_CP023633
• Organism: Mycobacterium tuberculosis strain TBDM2444

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CP490_RS03115
• Protein ID: WP_070895652.1
• Coordinates: 677848..678255 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O53778
• Locus tag: CP490_RS03110
• Protein ID: WP_003403039.1
• Coordinates: 677636..677851 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP490_RS03100	676164..676922	+	759	WP_003898511.1	Mut7-C ubiquitin/RNAse domain-containing protein	-
CP490_RS03105	677051..677542	-	492	WP_003403017.1	hypothetical protein	-
CP490_RS03110	677636..677851	+	216	WP_003403039.1	type II toxin-antitoxin system antitoxin VapB26	Antitoxin
CP490_RS03115	677848..678255	+	408	WP_070895652.1	type II toxin-antitoxin system toxin ribonuclease C26	Toxin
CP490_RS03120	678315..679001	-	687	WP_003403052.1	LpqN/LpqT family lipoprotein	-
CP490_RS03125	679155..681788	+	2634	WP_003403055.1	GH92 family glycosyl hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Ribbon-helix-helix protein, CopG (4-39)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14485.46 Da
• Isoelectric Point: 4.4493

>T85316 WP_070895652.1 NZ_CP023633:677848-678255 [Mycobacterium tuberculosis]
VIIDTSALLAYFDAAEPDHAAVSECIDSSADALVVSPYVVAELDYLVATRVGVDAELAVLRELAGGAWELANCGAAEIEQ
AARIVTKYQDQRIGIADAANVLLADRYRTRTILTLDRRHFSALRPIGGGRFTVIP

Nucleotide

• Length: 408 bp

>T85316 NZ_CP023633:677848-678255 [Mycobacterium tuberculosis]
GTGATCATCGACACGAGTGCGCTGCTTGCCTATTTCGACGCCGCCGAGCCAGACCACGCCGCAGTGTCTGAGTGCATCGA
TAGCTCCGCAGACGCGCTCGTCGTATCCCCTTATGTGGTAGCGGAACTCGACTATCTCGTCGCCACCCGGGTAGGTGTCG
ATGCCGAGCTCGCCGTCCTGCGTGAACTCGCCGGCGGGGCCTGGGAGCTCGCCAACTGCGGTGCCGCCGAAATCGAGCAG
GCCGCCCGCATCGTCACGAAATACCAGGATCAGCGGATCGGGATCGCGGATGCGGCCAACGTCTTGCTGGCCGACCGATA
CCGCACGCGCACGATCCTCACCCTGGACCGTCGGCACTTCTCGGCGCTGCGGCCGATCGGCGGTGGGCGCTTCACCGTCA
TTCCGTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7572.66 Da
• Isoelectric Point: 10.5454

>AT85316 WP_003403039.1 NZ_CP023633:677636-677851 [Mycobacterium tuberculosis]
VDKTTVYLPDELKAAVKRAARQRGVSEAQVIRESIRAAVGGAKPPPRGGLYAGSEPIARRVDELLAGFGER

Nucleotide

• Length: 216 bp

>AT85316 NZ_CP023633:677636-677851 [Mycobacterium tuberculosis]
GTGGACAAGACGACGGTCTACCTGCCGGATGAACTCAAGGCGGCCGTGAAGCGCGCCGCTCGGCAGCGCGGAGTCTCCGA
AGCGCAGGTAATCCGGGAGTCCATCCGGGCGGCGGTCGGCGGCGCCAAGCCGCCGCCGCGCGGGGGTCTATATGCGGGTT
CGGAGCCCATCGCGCGGCGAGTCGACGAGCTGCTGGCTGGCTTCGGTGAGCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BSA2