Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3182972..3183659 Replicon chromosome
Accession NZ_CP023632
Organism Mycobacterium tuberculosis strain TBDM2189

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag CP489_RS15680 Protein ID WP_003414624.1
Coordinates 3183216..3183659 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag CP489_RS15675 Protein ID WP_003414620.1
Coordinates 3182972..3183229 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP489_RS15655 3178292..3179146 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
CP489_RS15660 3179202..3180365 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
CP489_RS15665 3180382..3181596 - 1215 WP_003899518.1 zinc metalloprotease Rip -
CP489_RS15670 3181604..3182845 - 1242 WP_031658947.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
CP489_RS15675 3182972..3183229 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
CP489_RS15680 3183216..3183659 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
CP489_RS15685 3183739..3184401 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
CP489_RS15690 3184497..3184688 + 192 WP_031658948.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
CP489_RS15695 3185020..3186768 + 1749 WP_003912869.1 cytochrome c biogenesis protein DipZ -
CP489_RS15700 3186864..3187445 + 582 WP_003414644.1 fasciclin domain-containing protein -
CP489_RS15705 3187545..3187811 + 267 WP_015456449.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T85304 WP_003414624.1 NZ_CP023632:3183216-3183659 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T85304 NZ_CP023632:3183216-3183659 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT85304 WP_003414620.1 NZ_CP023632:3182972-3183229 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT85304 NZ_CP023632:3182972-3183229 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References