Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3109992..3110562 Replicon chromosome
Accession NZ_CP023632
Organism Mycobacterium tuberculosis strain TBDM2189

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag CP489_RS15310 Protein ID WP_003414166.1
Coordinates 3109992..3110348 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag CP489_RS15315 Protein ID WP_003901465.1
Coordinates 3110332..3110562 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP489_RS15290 3105444..3107132 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
CP489_RS15295 3107136..3107462 - 327 WP_003414157.1 hypothetical protein -
CP489_RS15300 3107635..3108222 + 588 WP_003914429.1 DUF3558 family protein -
CP489_RS15305 3108241..3109890 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
CP489_RS15310 3109992..3110348 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
CP489_RS15315 3110332..3110562 - 231 WP_003901465.1 antitoxin MazE Antitoxin
CP489_RS15320 3110605..3111648 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
CP489_RS15325 3111839..3112114 + 276 WP_003911993.1 DUF1778 domain-containing protein -
CP489_RS21540 3112290..3112544 - 255 WP_003917684.1 hypothetical protein -
CP489_RS15335 3112692..3113096 + 405 WP_009938577.1 hypothetical protein -
CP489_RS15340 3113093..3113284 + 192 WP_003414184.1 hypothetical protein -
CP489_RS15345 3113348..3114637 + 1290 Protein_2900 transposase family protein -
CP489_RS15350 3114871..3115128 + 258 WP_003899489.1 hypothetical protein -
CP489_RS15355 3115233..3115544 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T85300 WP_003414166.1 NZ_CP023632:c3110348-3109992 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T85300 NZ_CP023632:c3110348-3109992 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT85300 WP_003901465.1 NZ_CP023632:c3110562-3110332 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT85300 NZ_CP023632:c3110562-3110332 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References