Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2225252..2225940 Replicon chromosome
Accession NZ_CP023632
Organism Mycobacterium tuberculosis strain TBDM2189

Toxin (Protein)


Gene name vapC Uniprot ID P0A653
Locus tag CP489_RS10770 Protein ID WP_003409958.1
Coordinates 2225252..2225671 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag CP489_RS10775 Protein ID WP_003409968.1
Coordinates 2225680..2225940 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP489_RS10750 2220747..2221595 + 849 WP_003409942.1 class I SAM-dependent methyltransferase -
CP489_RS10755 2221558..2223003 - 1446 WP_003899115.1 APC family permease -
CP489_RS10760 2223182..2223868 - 687 WP_031658905.1 immunoprotective protein Mpt64 -
CP489_RS10765 2224059..2225027 - 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
CP489_RS10770 2225252..2225671 - 420 WP_003409958.1 type II toxin-antitoxin system VapC family toxin Toxin
CP489_RS10775 2225680..2225940 - 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CP489_RS10780 2226083..2227759 + 1677 WP_003899118.1 PecA family PE domain-processing aspartic protease -
CP489_RS10785 2227747..2228400 - 654 WP_003409976.1 cutinase Cfp21 -
CP489_RS10790 2228515..2228745 + 231 WP_003409980.1 DUF167 domain-containing protein -
CP489_RS10795 2228830..2229741 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
CP489_RS10800 2229850..2230449 + 600 WP_003409989.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14724.89 Da        Isoelectric Point: 7.9535

>T85283 WP_003409958.1 NZ_CP023632:c2225671-2225252 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Download         Length: 420 bp

>T85283 NZ_CP023632:c2225671-2225252 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT85283 WP_003409968.1 NZ_CP023632:c2225940-2225680 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT85283 NZ_CP023632:c2225940-2225680 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C4H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References