Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1946876..1947489 Replicon chromosome
Accession NZ_CP023632
Organism Mycobacterium tuberculosis strain TBDM2189

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag CP489_RS09370 Protein ID WP_003408465.1
Coordinates 1946876..1947265 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag CP489_RS09375 Protein ID WP_003408469.1
Coordinates 1947262..1947489 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP489_RS09340 1942505..1943419 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
CP489_RS09345 1943422..1944252 + 831 WP_003916363.1 cyclase family protein -
CP489_RS09350 1944252..1944602 + 351 WP_003898986.1 cupin domain-containing protein -
CP489_RS09355 1944655..1945473 + 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
CP489_RS09360 1945487..1946266 + 780 WP_003408460.1 IclR family transcriptional regulator -
CP489_RS09370 1946876..1947265 - 390 WP_003408465.1 PIN domain-containing protein Toxin
CP489_RS09375 1947262..1947489 - 228 WP_003408469.1 antitoxin Antitoxin
CP489_RS09380 1947707..1949191 + 1485 WP_003408473.1 carboxylase -
CP489_RS09385 1949188..1950435 + 1248 WP_003408476.1 serine hydrolase -
CP489_RS09390 1950478..1950897 - 420 WP_003408483.1 hypothetical protein -
CP489_RS09395 1950887..1951597 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T85278 WP_003408465.1 NZ_CP023632:c1947265-1946876 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T85278 NZ_CP023632:c1947265-1946876 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT85278 WP_003408469.1 NZ_CP023632:c1947489-1947262 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT85278 NZ_CP023632:c1947489-1947262 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References