Detailed information of TA system TA085262

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 752904..753537
• Replicon: chromosome
• Accession: NZ_CP023632
• Organism: Mycobacterium tuberculosis strain TBDM2189

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CP489_RS03530
• Protein ID: WP_070890539.1
• Coordinates: 752904..753287 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ56
• Locus tag: CP489_RS03535
• Protein ID: WP_003403368.1
• Coordinates: 753382..753537 (-)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP489_RS03505	748196..748732	+	537	WP_003403341.1	50S ribosomal protein L10	-
CP489_RS03510	748769..749161	+	393	WP_003403353.1	50S ribosomal protein L7/L12	-
CP489_RS03515	749154..749849	-	696	WP_003403355.1	TetR/AcrR family transcriptional regulator	-
CP489_RS03520	749920..751425	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
CP489_RS03525	751506..752516	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
CP489_RS03530	752904..753287	-	384	WP_070890539.1	VapC toxin family PIN domain ribonuclease	Toxin
CP489_RS03535	753382..753537	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CP489_RS03540	753613..754329	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
CP489_RS03545	754605..754913	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
CP489_RS03550	754900..755145	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
CP489_RS03555	755255..755692	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
CP489_RS03560	755689..755943	-	255	WP_003911263.1	antitoxin VapB7	-
CP489_RS03565	756057..758420	+	2364	WP_003898547.1	arylsulfatase AtsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13945.70 Da
• Isoelectric Point: 6.0803

>T85262 WP_070890539.1 NZ_CP023632:c753287-752904 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLVCDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Nucleotide

• Length: 384 bp

>T85262 NZ_CP023632:c753287-752904 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGGTCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5872.72 Da
• Isoelectric Point: 5.7734

>AT85262 WP_003403368.1 NZ_CP023632:c753537-753382 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Nucleotide

• Length: 156 bp

>AT85262 NZ_CP023632:c753537-753382 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U4BSF8