Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3109948..3110518 Replicon chromosome
Accession NZ_CP023631
Organism Mycobacterium tuberculosis strain TBDM1506

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag CP487_RS15305 Protein ID WP_003414166.1
Coordinates 3109948..3110304 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag CP487_RS15310 Protein ID WP_003901465.1
Coordinates 3110288..3110518 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP487_RS15285 3105400..3107088 - 1689 WP_010924579.1 alpha/beta hydrolase family protein -
CP487_RS15290 3107092..3107418 - 327 WP_003414157.1 hypothetical protein -
CP487_RS15295 3107591..3108178 + 588 WP_003914429.1 DUF3558 family protein -
CP487_RS15300 3108197..3109846 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
CP487_RS15305 3109948..3110304 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
CP487_RS15310 3110288..3110518 - 231 WP_003901465.1 antitoxin MazE Antitoxin
CP487_RS15315 3110561..3111604 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
CP487_RS15320 3111795..3112070 + 276 WP_003911993.1 DUF1778 domain-containing protein -
CP487_RS21510 3112246..3112500 - 255 WP_003917684.1 hypothetical protein -
CP487_RS15330 3112648..3113052 + 405 WP_009938577.1 hypothetical protein -
CP487_RS15335 3113049..3113240 + 192 WP_003414184.1 hypothetical protein -
CP487_RS15340 3113304..3114593 + 1290 Protein_2900 transposase family protein -
CP487_RS15345 3114827..3115084 + 258 WP_003899489.1 hypothetical protein -
CP487_RS15350 3115189..3115500 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T85239 WP_003414166.1 NZ_CP023631:c3110304-3109948 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T85239 NZ_CP023631:c3110304-3109948 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT85239 WP_003901465.1 NZ_CP023631:c3110518-3110288 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT85239 NZ_CP023631:c3110518-3110288 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References