Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3069954..3070633 Replicon chromosome
Accession NZ_CP023631
Organism Mycobacterium tuberculosis strain TBDM1506

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag CP487_RS15080 Protein ID WP_003414059.1
Coordinates 3069954..3070370 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag CP487_RS15085 Protein ID WP_003414061.1
Coordinates 3070367..3070633 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP487_RS15060 3066006..3066908 - 903 WP_031665916.1 4-hydroxy-tetrahydrodipicolinate synthase -
CP487_RS15065 3066977..3067729 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
CP487_RS15070 3067973..3068248 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
CP487_RS15075 3068245..3069867 - 1623 WP_003414057.1 type I restriction-modification system subunit M -
CP487_RS15080 3069954..3070370 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
CP487_RS15085 3070367..3070633 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CP487_RS15090 3070659..3071054 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
CP487_RS15095 3071051..3071320 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
CP487_RS15100 3071330..3072424 - 1095 WP_003414068.1 restriction endonuclease subunit S -
CP487_RS15105 3072421..3072840 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
CP487_RS15110 3072914..3073393 - 480 WP_003414073.1 dihydrofolate reductase -
CP487_RS15115 3073464..3074264 - 801 WP_003911953.1 thymidylate synthase -
CP487_RS15120 3074420..3075157 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-123)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T85237 WP_003414059.1 NZ_CP023631:c3070370-3069954 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T85237 NZ_CP023631:c3070370-3069954 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT85237 WP_003414061.1 NZ_CP023631:c3070633-3070367 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT85237 NZ_CP023631:c3070633-3070367 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5SV2


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References