Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2868387..2869018 Replicon chromosome
Accession NZ_CP023631
Organism Mycobacterium tuberculosis strain TBDM1506

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag CP487_RS13940 Protein ID WP_003413174.1
Coordinates 2868641..2869018 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag CP487_RS13935 Protein ID WP_003413167.1
Coordinates 2868387..2868644 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP487_RS13900 2864301..2864615 + 315 WP_009937839.1 hypothetical protein -
CP487_RS13905 2864911..2866122 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
CP487_RS13915 2866249..2866908 + 660 WP_003900846.1 LppA family lipoprotein -
CP487_RS13920 2866905..2867567 + 663 WP_003900848.1 hypothetical protein -
CP487_RS13925 2867564..2867842 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
CP487_RS13930 2867935..2868348 + 414 WP_003413164.1 PIN domain nuclease -
CP487_RS13935 2868387..2868644 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
CP487_RS13940 2868641..2869018 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
CP487_RS13945 2869034..2869408 - 375 WP_003413177.1 hypothetical protein -
CP487_RS13950 2869508..2869903 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
CP487_RS13955 2869900..2870145 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
CP487_RS13965 2870556..2870975 - 420 WP_003413190.1 A24 family peptidase -
CP487_RS13970 2870987..2871796 - 810 WP_003413193.1 shikimate dehydrogenase -
CP487_RS13975 2871793..2873049 - 1257 WP_014585306.1 endolytic transglycosylase MltG -
CP487_RS13980 2873042..2873554 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T85234 WP_003413174.1 NZ_CP023631:2868641-2869018 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T85234 NZ_CP023631:2868641-2869018 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT85234 WP_003413167.1 NZ_CP023631:2868387-2868644 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT85234 NZ_CP023631:2868387-2868644 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References