Detailed information of TA system TA085211

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1239278..1239846
• Replicon: chromosome
• Accession: NZ_CP023631
• Organism: Mycobacterium tuberculosis strain TBDM1506

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: CP487_RS06085
• Protein ID: WP_003405865.1
• Coordinates: 1239472..1239846 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: CP487_RS06080
• Protein ID: WP_003405863.1
• Coordinates: 1239278..1239475 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP487_RS06060	1235319..1235957	-	639	WP_003898730.1	lipid droplet-associated protein	-
CP487_RS06065	1236047..1237054	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
CP487_RS06070	1237071..1238054	-	984	WP_003898731.1	hypothetical protein	-
CP487_RS06075	1238117..1239190	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
CP487_RS06080	1239278..1239475	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CP487_RS06085	1239472..1239846	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP487_RS06090	1240049..1240747	+	699	WP_003898733.1	hypothetical protein	-
CP487_RS06095	1240865..1241050	+	186	WP_003901093.1	hypothetical protein	-
CP487_RS06100	1240977..1241252	-	276	WP_003405867.1	hypothetical protein	-
CP487_RS06105	1241495..1241818	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
CP487_RS06110	1241833..1242543	-	711	WP_003911399.1	hypothetical protein	-
CP487_RS21710	1242565..1242774	+	210	WP_003911400.1	hypothetical protein	-
CP487_RS06115	1242726..1243433	-	708	Protein_1162	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T85211 WP_003405865.1 NZ_CP023631:1239472-1239846 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T85211 NZ_CP023631:1239472-1239846 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT85211 WP_003405863.1 NZ_CP023631:1239278-1239475 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT85211 NZ_CP023631:1239278-1239475 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07