Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3707544..3708218 Replicon chromosome
Accession NZ_CP023630
Organism Mycobacterium tuberculosis strain MDRMA2441

Toxin (Protein)


Gene name vapC Uniprot ID P9WF52
Locus tag CP486_RS18190 Protein ID WP_003417282.1
Coordinates 3707544..3707972 (-) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TI59
Locus tag CP486_RS18195 Protein ID WP_003417286.1
Coordinates 3707976..3708218 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP486_RS18165 3703366..3703767 - 402 WP_003417264.1 cytidine deaminase -
CP486_RS18170 3703908..3704342 + 435 WP_003900017.1 succinate dehydrogenase, cytochrome b556 subunit -
CP486_RS18175 3704339..3704773 + 435 WP_003417269.1 succinate dehydrogenase hydrophobic membrane anchor subunit -
CP486_RS18180 3704902..3706674 + 1773 WP_003417273.1 succinate dehydrogenase flavoprotein subunit -
CP486_RS18185 3706674..3707465 + 792 WP_003417279.1 succinate dehydrogenase iron-sulfur subunit -
CP486_RS18190 3707544..3707972 - 429 WP_003417282.1 type II toxin-antitoxin system VapC family toxin Toxin
CP486_RS18195 3707976..3708218 - 243 WP_003417286.1 ribbon-helix-helix domain-containing protein Antitoxin
CP486_RS18200 3708340..3708954 - 615 WP_003417288.1 class I SAM-dependent methyltransferase -
CP486_RS18205 3708951..3709616 - 666 WP_003417290.1 molybdopterin converting factor subunit 1 -
CP486_RS18210 3709617..3710150 - 534 WP_003417293.1 cyclic pyranopterin monophosphate synthase MoaC -
CP486_RS21945 3710147..3710299 - 153 WP_003916129.1 hypothetical protein -
CP486_RS18220 3710335..3711596 + 1262 WP_096865988.1 IS3-like element IS987 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-141)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15834.16 Da        Isoelectric Point: 8.5471

>T85185 WP_003417282.1 NZ_CP023630:c3707972-3707544 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Download         Length: 429 bp

>T85185 NZ_CP023630:c3707972-3707544 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8849.86 Da        Isoelectric Point: 5.1007

>AT85185 WP_003417286.1 NZ_CP023630:c3708218-3707976 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Download         Length: 243 bp

>AT85185 NZ_CP023630:c3708218-3707976 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBL0

Antitoxin

Source ID Structure
AlphaFold DB G0TI59

References