Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3183055..3183742 Replicon chromosome
Accession NZ_CP023630
Organism Mycobacterium tuberculosis strain MDRMA2441

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag CP486_RS15700 Protein ID WP_003414624.1
Coordinates 3183299..3183742 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag CP486_RS15695 Protein ID WP_003414620.1
Coordinates 3183055..3183312 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP486_RS15675 3178375..3179229 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
CP486_RS15680 3179285..3180448 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
CP486_RS15685 3180465..3181679 - 1215 WP_003899518.1 zinc metalloprotease Rip -
CP486_RS15690 3181687..3182928 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
CP486_RS15695 3183055..3183312 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
CP486_RS15700 3183299..3183742 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
CP486_RS15705 3183822..3184484 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
CP486_RS15710 3184580..3184771 + 192 WP_003414632.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
CP486_RS15715 3185103..3186851 + 1749 WP_003912869.1 cytochrome c biogenesis protein DipZ -
CP486_RS15720 3186947..3187528 + 582 WP_003414644.1 fasciclin domain-containing protein -
CP486_RS15725 3187628..3187894 + 267 WP_015456449.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T85183 WP_003414624.1 NZ_CP023630:3183299-3183742 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T85183 NZ_CP023630:3183299-3183742 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT85183 WP_003414620.1 NZ_CP023630:3183055-3183312 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT85183 NZ_CP023630:3183055-3183312 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References